Effective and low-cost method to obtain purified C-phycocyanins from Arthrospira platensis

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发表日期：

2025年3月22日

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Ingrid Rocha Teixeira,
Giovana Domeneghini Mercali,
Débora Pez Jaeschke,
Ligia Damasceno Ferreira Marczak

摘要

Purification of phycocyanin extracts from Arthrospira platensis was evaluated by saline precipitation ((NH₄)₂SO₄, 20–80% saturation) and affinity purification with activated carbon (10–120, g·L⁻¹) and chitosan (1—30, g·L⁻¹). Moreover, different combinations of these techniques, along with membrane filtration (polyvinylidene fluoride, 0.45 µm), were explored. For saline precipitation, greatest purity values (1.5) and phycocyanin content (50 mg g⁻¹) in the precipitate were achieved with 50% saturation (NH₄)₂SO₄. Regarding affinity purification, the highest purity (1.71) was obtained with 65 g·L⁻¹ of activated carbon and 1 g·L⁻¹ of chitosan. Superior purity results were obtained by affinity purification with activated carbon (120 g·L⁻¹) followed by salt precipitation ((NH₄)₂SO₄, 50% saturation) and membrane filtration. This combined strategy led to an increase in purity from 1.16 to 2.52. However, there was a 50% reduction in phycocyanin recovery after the first two steps, with no significant difference observed upon the addition of filtration.