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<p>This study investigates the test–retest reliability of exhaled acetone and isoprene as potential biomarkers for monitoring exercise-induced physiological changes. Ten healthy active males (age 22.7 ± 3.7&#xa0;years, height 182.4 ± 6.8&#xa0;cm, body mass 80.12 ± 8.5&#xa0;kg, body mass index 24.0 ± 1.4&#xa0;kg/m<sup>2</sup>, VO<sub>2peak</sub> 55 ± 4&#xa0;mL/kg/min, physical activity 322&#xa0;min/week) provided baseline measures before undergoing a VO<sub>2peak</sub> test and two treadmill exercise trials, interspersed by 7-days’ rest. Treadmill exercise consisted of 20&#xa0;min at 80% of VO<sub>2peak</sub> speed. Breath samples were collected before exercise, 10&#xa0;min, and 24&#xa0;h after exercise, using glass containers and solid-phase microextraction fibres, and analysed by gas-chromatography mass-spectrometry. Exhaled acetone exhibited poor internal consistency (Cronbach’s <i>α</i> = −&#xa0;0.184) and agreement (<i>ICC</i> = −&#xa0;0.096 [−&#xa0;0.836–0.607], <i>P</i> = 0.592) with a high technical error (TE = 2.38&#xa0;×&#xa0;10<sup>6</sup>) and coefficient of variation (<i>CV</i> = 90%). In contrast, exhaled isoprene provided satisfactory internal consistency (Cronbach’s <i>α</i> 0.817) and good agreement (<i>ICC</i>&#xa0;= 0.699, <i>P</i> = 0.009), with a lower technical error (TE = 1.24&#xa0;×&#xa0;10<sup>6</sup>) and coefficient of variation (<i>CV</i> = 28%). Quadratic regression showed a decrease in isoprene 10&#xa0;min after exercise during trial 1 (<i>P</i> &lt; 0.01) and trial 2 (<i>P</i> &lt; 0.05) and returned to baseline levels after 24&#xa0;h. While acetone’s reliability was questionable, isoprene levels following 20&#xa0;min of submaximal treadmill exercise are notably reproducible among healthy active males.</p>

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Reliability of Exhaled Acetone and Isoprene in Healthy Active Adult Responses to Sub-maximal Treadmill Exercise: A Pilot Study

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月8日

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  • Leo R. Bell,
  • Mark A. Myers,
  • Jack T. Harvey,
  • Declan Hennessy,
  • Ryan L. Worn,
  • Matthew P. Wallen,
  • Greg Davis,
  • Brendan J. O’Brien

摘要

This study investigates the test–retest reliability of exhaled acetone and isoprene as potential biomarkers for monitoring exercise-induced physiological changes. Ten healthy active males (age 22.7 ± 3.7 years, height 182.4 ± 6.8 cm, body mass 80.12 ± 8.5 kg, body mass index 24.0 ± 1.4 kg/m2, VO2peak 55 ± 4 mL/kg/min, physical activity 322 min/week) provided baseline measures before undergoing a VO2peak test and two treadmill exercise trials, interspersed by 7-days’ rest. Treadmill exercise consisted of 20 min at 80% of VO2peak speed. Breath samples were collected before exercise, 10 min, and 24 h after exercise, using glass containers and solid-phase microextraction fibres, and analysed by gas-chromatography mass-spectrometry. Exhaled acetone exhibited poor internal consistency (Cronbach’s α = − 0.184) and agreement (ICC = − 0.096 [− 0.836–0.607], P = 0.592) with a high technical error (TE = 2.38 × 106) and coefficient of variation (CV = 90%). In contrast, exhaled isoprene provided satisfactory internal consistency (Cronbach’s α 0.817) and good agreement (ICC = 0.699, P = 0.009), with a lower technical error (TE = 1.24 × 106) and coefficient of variation (CV = 28%). Quadratic regression showed a decrease in isoprene 10 min after exercise during trial 1 (P < 0.01) and trial 2 (P < 0.05) and returned to baseline levels after 24 h. While acetone’s reliability was questionable, isoprene levels following 20 min of submaximal treadmill exercise are notably reproducible among healthy active males.