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<p><i>Septoria tritici</i> blotch, caused by <i>Zymoseptoria tritici</i>, is a major constraint to wheat production in Mediterranean environments. We performed a genome-wide association study-GWAS on 191 elite ICARDA spring bread wheat genotypes evaluated under natural field conditions in two Moroccan environments (Sidi Allal Tazi and Marchouch). Disease severity at the adult plant stage was assessed using standard scoring and the area under the disease progress curve (AUDPC). Genotyping was conducted using the Illumina 15&#xa0;K SNP array, and association analyses were performed using mixed and multi-locus models implemented in TASSEL and GAPIT. Substantial phenotypic variation was observed among genotypes. Variance components estimated using a multi-environment mixed model revealed a moderate broad-sense heritability (<i>H²</i> = 0.65) for STB resistance across environments. Four genotypes showed stable resistance across both locations. GWAS identified 35 marker-trait associations distributed across multiple chromosomes. Two major QTLs, <i>qSTB.01</i> on chromosome 5D (548–550&#xa0;Mb) and <i>qSTB.02</i> on chromosome 5B (~ 473&#xa0;Mb), were consistently detected across models and environments. The lead SNPs BobWhite_c7604_181 and wsnp_Ex_c974_1864971 explained up to 24.2% and 51.9% of the phenotypic variance, respectively. Several loci co-localized with previously reported regions, while additional loci were detected on chromosomes 7&#xa0;A, 6B, and 7D. Putative candidate within the identified QTL intervals included genes encoding NB-ARC domain proteins, ABC transporters, mitogen-activated protein kinases, and peroxidases within QTL intervals. These findings identify genomic regions associated with STB resistance and provide targets for marker-assisted selection in wheat breeding.</p>

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Genome-wide association mapping of adult plant resistance to Septoria tritici blotch in spring bread wheat

  • 期刊论文

  • 发表日期：

    2026年7月16日

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  • Fatima Zahra Mssilea,
  • Fatima Henkrar,
  • Khaoula Lahrichi,
  • Sabah El Mrabti,
  • Hicham Derboune,
  • Wuletaw Tadesse

摘要

Septoria tritici blotch, caused by Zymoseptoria tritici, is a major constraint to wheat production in Mediterranean environments. We performed a genome-wide association study-GWAS on 191 elite ICARDA spring bread wheat genotypes evaluated under natural field conditions in two Moroccan environments (Sidi Allal Tazi and Marchouch). Disease severity at the adult plant stage was assessed using standard scoring and the area under the disease progress curve (AUDPC). Genotyping was conducted using the Illumina 15 K SNP array, and association analyses were performed using mixed and multi-locus models implemented in TASSEL and GAPIT. Substantial phenotypic variation was observed among genotypes. Variance components estimated using a multi-environment mixed model revealed a moderate broad-sense heritability (H² = 0.65) for STB resistance across environments. Four genotypes showed stable resistance across both locations. GWAS identified 35 marker-trait associations distributed across multiple chromosomes. Two major QTLs, qSTB.01 on chromosome 5D (548–550 Mb) and qSTB.02 on chromosome 5B (~ 473 Mb), were consistently detected across models and environments. The lead SNPs BobWhite_c7604_181 and wsnp_Ex_c974_1864971 explained up to 24.2% and 51.9% of the phenotypic variance, respectively. Several loci co-localized with previously reported regions, while additional loci were detected on chromosomes 7 A, 6B, and 7D. Putative candidate within the identified QTL intervals included genes encoding NB-ARC domain proteins, ABC transporters, mitogen-activated protein kinases, and peroxidases within QTL intervals. These findings identify genomic regions associated with STB resistance and provide targets for marker-assisted selection in wheat breeding.