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<p>Brown planthopper, <i>Nilaparvata lugens</i> (Stål), poses a significant threat to rice cultivation, highlighting the need to identify broad-spectrum resistance genes. To elucidate the genetic basis of BPH resistance, a mapping population of 260 progenies derived from a cross between <i>Oryza glaberrima</i> accession IR75870 and a susceptible indica variety, Improved Samba Mahsuri (ISM) was screened against BPH Biotype 4. Inheritance study based on damage scores from BPH infestation at the seedling stage revealed 1:3 segregation ratio confirming that BPH resistance is governed by a single recessive gene. Bulked segregant analysis (BSA) using 393 SSR markers identified 71 polymorphic markers (18.07% polymorphism) between the parents. Screening of these markers across resistant and susceptible bulks localized the resistance locus to chromosome 12. Four SSR markers—RM7018, RM27970, RM2851, and RM519—showed linkage to the resistance gene. Genotyping of the F₅ mapping population followed by QTL analysis using ICIM software identified a major QTL, <i>bph52(t)</i> on the long arm of chromosome 12, flanked by markers RM519 and RM27970, with an LOD score of 23.066. This QTL explained 36.32% of the phenotypic variance for BPH resistance, with a map distance of 20.34&#xa0;cM between the flanking markers. Further research is needed to saturate the marker interval with more closely linked markers. The identified F<sub>6</sub> pre-breeding lines hold promise for developing BPH-resistant rice cultivars.</p>

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Identification of a novel locus conferring resistance to brown planthopper (Nilaparvata lugens (Stål.) from Oryza glaberrima Steud.

  • 期刊论文

  • 发表日期：

    2026年4月18日

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  • Neha Chhabra,
  • Pavneet Kaur,
  • Preetinder Singh Sarao,
  • Kumari Neelam,
  • R. M. Sundaram

摘要

Brown planthopper, Nilaparvata lugens (Stål), poses a significant threat to rice cultivation, highlighting the need to identify broad-spectrum resistance genes. To elucidate the genetic basis of BPH resistance, a mapping population of 260 progenies derived from a cross between Oryza glaberrima accession IR75870 and a susceptible indica variety, Improved Samba Mahsuri (ISM) was screened against BPH Biotype 4. Inheritance study based on damage scores from BPH infestation at the seedling stage revealed 1:3 segregation ratio confirming that BPH resistance is governed by a single recessive gene. Bulked segregant analysis (BSA) using 393 SSR markers identified 71 polymorphic markers (18.07% polymorphism) between the parents. Screening of these markers across resistant and susceptible bulks localized the resistance locus to chromosome 12. Four SSR markers—RM7018, RM27970, RM2851, and RM519—showed linkage to the resistance gene. Genotyping of the F₅ mapping population followed by QTL analysis using ICIM software identified a major QTL, bph52(t) on the long arm of chromosome 12, flanked by markers RM519 and RM27970, with an LOD score of 23.066. This QTL explained 36.32% of the phenotypic variance for BPH resistance, with a map distance of 20.34 cM between the flanking markers. Further research is needed to saturate the marker interval with more closely linked markers. The identified F6 pre-breeding lines hold promise for developing BPH-resistant rice cultivars.