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<p>Tan spot disease, caused by <i>Pyrenophora tritici-repentis</i>, poses a substantial threat to global wheat crops, resulting in considerable economic losses. The effective utilization of dis-ease-resistant wheat genotypes relies on a thorough understanding of the genetic makeup of the pathogen, particularly the prevalence of key virulence factors within the fungal population. This study aimed to assess the reactions of 48 wheat genotypes to ToxA toxin infiltration, the primary pathogenic factor of <i>P. tritici-repentis</i>. The findings indicated that 23 genotypes, including the ToxA-sensitive Grandin genotype employed as a control, exhibited necrotic symptoms 48&#xa0;h post ToxA infiltration, classifying them as ToxA-sensitive lines. Conversely, the remaining genotypes demonstrated resistance to ToxA, categorizing them as ToxA-resistant lines. To validate these observations, a PCR assay was conducted using specific primers targeting the Tox-sensitivity gene <i>Tsn1</i>. The results indicated successful amplification of the 380&#xa0;bp band in the sensitive lines, confirming the presence of the Tox-sensitivity gene Tsn1. In contrast, all genotypes resistant to ToxA infiltration failed to amplify the Tsn1 gene, consistent with the outcomes of toxin infiltration. This underscores the proposed approach as a preliminary screening tool for wheat genotypes, employing negative selection to identify and exclude sensitive genotypes from breeding initiatives. In summary, this study emphasizes the efficacy of the proposed method in screening wheat genotypes, offering a valuable tool for the early identification of sensitive lines and informing breeding programs dedicated to developing resistance against tan spot disease.</p>

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Application of Tsn1 molecular marker for the differentiation of Tan spot (Pyrenophora tritici-repentis) resistance in wheat cultivars

  • 期刊论文

  • 发表日期：

    2026年7月9日

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  • Rahim Mehrabi,
  • Tayebeh Bakhshi,
  • Mostafa Aghaee Sarbarzeh,
  • Aras Türkoğlu,
  • Kamil Haliloğlu,
  • Farajollah Shahriari Ahmadi,
  • Alireza Seifi

摘要

Tan spot disease, caused by Pyrenophora tritici-repentis, poses a substantial threat to global wheat crops, resulting in considerable economic losses. The effective utilization of dis-ease-resistant wheat genotypes relies on a thorough understanding of the genetic makeup of the pathogen, particularly the prevalence of key virulence factors within the fungal population. This study aimed to assess the reactions of 48 wheat genotypes to ToxA toxin infiltration, the primary pathogenic factor of P. tritici-repentis. The findings indicated that 23 genotypes, including the ToxA-sensitive Grandin genotype employed as a control, exhibited necrotic symptoms 48 h post ToxA infiltration, classifying them as ToxA-sensitive lines. Conversely, the remaining genotypes demonstrated resistance to ToxA, categorizing them as ToxA-resistant lines. To validate these observations, a PCR assay was conducted using specific primers targeting the Tox-sensitivity gene Tsn1. The results indicated successful amplification of the 380 bp band in the sensitive lines, confirming the presence of the Tox-sensitivity gene Tsn1. In contrast, all genotypes resistant to ToxA infiltration failed to amplify the Tsn1 gene, consistent with the outcomes of toxin infiltration. This underscores the proposed approach as a preliminary screening tool for wheat genotypes, employing negative selection to identify and exclude sensitive genotypes from breeding initiatives. In summary, this study emphasizes the efficacy of the proposed method in screening wheat genotypes, offering a valuable tool for the early identification of sensitive lines and informing breeding programs dedicated to developing resistance against tan spot disease.