Scholarlink

<p>Number of Grains1 (<i>NOG1</i>), which encodes an enoyl-CoA hydratase/isomerase of the fatty acid β-oxidation pathway on chromosome 1, positively regulates the grain number in rice without affecting grain weight or panicle number. One favourable allelic variant of NOG1, with two copies of a 12-bp insertion in the promoter region at 2214&#xa0;bp upstream of the translation start site, from the Indica cultivar, has been reported to positively regulate grain number compared with others with a single copy of the 12-bp stretch. This study examined the status of NOG1 in 200 genotypes and developed a PCR-based, easy-to-use, co-dominant INDEL marker that distinguishes among genotypes with single or two copies of a 12&#xa0;bp insertion. Further validation of this marker has been confirmed in RILs and NILs developed from a cross between Bidhan Suruchi (with two copies of the insertion) and Nagina 22 (with a single copy of the insertion). Genotypes and NILs with the Bidhan Suruchi type NOG1 allele also showed a higher relative abundance of the <i>NOG1</i> transcript in leaf tissues of 21-day-old seedlings. This newly identified, validated NOG1 INDEL marker in the Indian germplasm will accelerate MAS to precisely improve rice yield.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Identification of allelic variation of NOG1 and development of an INDEL-based co-dominant marker for enhancing grain number in rice

  • 期刊论文

  • 发表日期：

    2026年2月4日

查看全文
  • Purkaystha S,
  • Sen P,
  • Saha AK,
  • Das D,
  • Saha S,
  • Ganguly S,
  • Bhattacharyya PK,
  • Bhattacharyya S

摘要

Number of Grains1 (NOG1), which encodes an enoyl-CoA hydratase/isomerase of the fatty acid β-oxidation pathway on chromosome 1, positively regulates the grain number in rice without affecting grain weight or panicle number. One favourable allelic variant of NOG1, with two copies of a 12-bp insertion in the promoter region at 2214 bp upstream of the translation start site, from the Indica cultivar, has been reported to positively regulate grain number compared with others with a single copy of the 12-bp stretch. This study examined the status of NOG1 in 200 genotypes and developed a PCR-based, easy-to-use, co-dominant INDEL marker that distinguishes among genotypes with single or two copies of a 12 bp insertion. Further validation of this marker has been confirmed in RILs and NILs developed from a cross between Bidhan Suruchi (with two copies of the insertion) and Nagina 22 (with a single copy of the insertion). Genotypes and NILs with the Bidhan Suruchi type NOG1 allele also showed a higher relative abundance of the NOG1 transcript in leaf tissues of 21-day-old seedlings. This newly identified, validated NOG1 INDEL marker in the Indian germplasm will accelerate MAS to precisely improve rice yield.