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<p>Rice is a staple food crop globally, with its demand increasing steadily each year. Rice production is increasingly threatened by biotic stresses, among which bakanae disease predominantly caused by <i>Fusarium fujikuroi</i> poses a significant challenge, leading to progressive yield losses in major rice-producing countries, including India. In this study, 927 rice landraces were screened for bakanae resistance under artificially controlled and field conditions in <i>Kharif</i> season (June–November) for two consecutive years (2021–2022). Based on phenotypic assessments, twenty genotypes exhibiting varying degree of resistance were further confirmed at Kashmir and New Delhi and were also selected for further studies. Biochemical assays, viz. phenylalanine ammonia lyase<i>,</i> β-1,3-glucanase, chitinase, and catalase, were performed for the selected genotypes. Among these genotypes showing higher enzymatic activities were further selected and evaluated for the pathogen colonization using <i>Fusarium fujikuroi</i>-specific marker (GenBank accession KY953210). Relative gene expression analysis for defense-related genes (<i>pvpal</i>, <i>pvch5b</i>, and <i>catalase</i>) was also carried out. Based on qPCR results, landraces with Indigenous collection number, 465304 and 513704, were identified to be resistant to bakanae, as indicated by significantly low copy number of pathogen-specific gene and high expression levels of defense related genes. Henceforth, these resistant landraces identified can be utilized in bakanae resistance breeding programs for the development of bakanae-resistant rice varieties and management of bakanae disease.</p>

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Unraveling the novel sources of resistance in rice landraces against an emerging bakanae disease through phenotypic, biochemical, and gene expression studies

  • 期刊论文

  • 发表日期：

    2025年10月16日

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  • Shashi Pandey,
  • Ayush Raj Singh,
  • Asif Bashir Shikari,
  • Gaurav Kumar Yadav,
  • S. Gopala Krishnan,
  • Ashok Kumar Singh,
  • Soumya Sharma,
  • Sapna Sharma,
  • Ranjith K. Ellur,
  • Prolay K. Bhowmick,
  • Deepak Ganjewala,
  • Bishnu Maya Bashyal

摘要

Rice is a staple food crop globally, with its demand increasing steadily each year. Rice production is increasingly threatened by biotic stresses, among which bakanae disease predominantly caused by Fusarium fujikuroi poses a significant challenge, leading to progressive yield losses in major rice-producing countries, including India. In this study, 927 rice landraces were screened for bakanae resistance under artificially controlled and field conditions in Kharif season (June–November) for two consecutive years (2021–2022). Based on phenotypic assessments, twenty genotypes exhibiting varying degree of resistance were further confirmed at Kashmir and New Delhi and were also selected for further studies. Biochemical assays, viz. phenylalanine ammonia lyase, β-1,3-glucanase, chitinase, and catalase, were performed for the selected genotypes. Among these genotypes showing higher enzymatic activities were further selected and evaluated for the pathogen colonization using Fusarium fujikuroi-specific marker (GenBank accession KY953210). Relative gene expression analysis for defense-related genes (pvpal, pvch5b, and catalase) was also carried out. Based on qPCR results, landraces with Indigenous collection number, 465304 and 513704, were identified to be resistant to bakanae, as indicated by significantly low copy number of pathogen-specific gene and high expression levels of defense related genes. Henceforth, these resistant landraces identified can be utilized in bakanae resistance breeding programs for the development of bakanae-resistant rice varieties and management of bakanae disease.