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<p>Hybrid rice technology provides over 20% higher yields than pure line varieties, relying on the development of superior parental lines for success. In this study successful marker-assisted introgression of three key bacterial leaf blight (BLB) resistance genes <i>Xa21,</i> <i>xa13</i>&#xa0;and&#xa0;<i>xa5</i>&#xa0;into elite restorer RP5933-1–19-2R for hybrid development. True F₁ plants harbouring all target genes were identified using gene-specific markers, and subsequent backcrossing was employed to develop BC₁F₁ and BC₂F₁ generations. Foreground selection coupled with background selection using identified 73 polymorphic SSR markers between parents, facilitated the recovery of up to 93.2% of the recurrent parent genome in selected BC₂F₁ plants. Field evaluations demonstrated that the improved BC<sub>2</sub>F<sub>3</sub>&#xa0;lines exhibited significantly enhanced resistance to BLB, scoring between 1 and 3 on the disease severity scale, compared to the susceptible check. In addition, agronomic assessments revealed that the improved lines maintained or exceeded the grain yield and desirable traits of the recurrent parent, viz., days to flowering, plant height and panicle length. The introgressed lines, carrying the major fertility restorer gene&#xa0;<i>Rf4</i>&#xa0;along with BLB resistance genes, also proved to be an effective restorer while crossed with WA-CMS lines and demonstrated superior yield heterosis. The improved BLB-resistant restorer lines through marker-assisted backcross breeding for developing new rice hybrids are highly beneficial and would contribute to sustainable rice production and enhanced food security.</p>

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Improving Parental lines for Bacterial Blight Resistance: Accelerating Rice Hybrid Development through Marker-Assisted Backcross Breeding (MABB)

  • 期刊论文

  • 发表日期：

    2025年9月19日

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  • Edukondalu Bandela,
  • Gourishankar Vuppu,
  • Sandeep G,
  • Soujanya Thota,
  • Mondem Bhargavi,
  • GouriSankar Laha,
  • Saida Naik D,
  • Srinivasa Chary D,
  • Meenakshi Sundaram Raman,
  • Revathi Ponnuswamy

摘要

Hybrid rice technology provides over 20% higher yields than pure line varieties, relying on the development of superior parental lines for success. In this study successful marker-assisted introgression of three key bacterial leaf blight (BLB) resistance genes Xa21, xa13 and xa5 into elite restorer RP5933-1–19-2R for hybrid development. True F₁ plants harbouring all target genes were identified using gene-specific markers, and subsequent backcrossing was employed to develop BC₁F₁ and BC₂F₁ generations. Foreground selection coupled with background selection using identified 73 polymorphic SSR markers between parents, facilitated the recovery of up to 93.2% of the recurrent parent genome in selected BC₂F₁ plants. Field evaluations demonstrated that the improved BC2F3 lines exhibited significantly enhanced resistance to BLB, scoring between 1 and 3 on the disease severity scale, compared to the susceptible check. In addition, agronomic assessments revealed that the improved lines maintained or exceeded the grain yield and desirable traits of the recurrent parent, viz., days to flowering, plant height and panicle length. The introgressed lines, carrying the major fertility restorer gene Rf4 along with BLB resistance genes, also proved to be an effective restorer while crossed with WA-CMS lines and demonstrated superior yield heterosis. The improved BLB-resistant restorer lines through marker-assisted backcross breeding for developing new rice hybrids are highly beneficial and would contribute to sustainable rice production and enhanced food security.