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<p>Cytoplasmic male sterility (CMS) lines are crucial for exploiting wheat heterosis and producing hybrid seeds. MicroRNAs (miRNAs), key endogenous non-coding small RNAs in plant growth and development, are understudied in regulating wheat male sterility. This study employed comparative small RNA sequencing to analyze the anther of the CMS line “S1376A” and its maintainer line “1376B” using high-throughput sequencing. A total of 114 known miRNAs belonging to 2473 conserved and 364 non-conserved miRNA families were isolated, and 2837 potential novel miRNA families were identified for the first time in floral buds of wheat. Multiple miRNA target genes associated with CMS occurrence encode essential transcription factors and proteins, likely involved in biological processes like starch and sucrose metabolism regulation, MYB expression, glycolysis, and microtubule formation. In “S1376A,” sucrose synthase and phosphate synthase activities were lower than in “1376B”; starch metabolism enzymes (α-amylase, β-amylase, etc.) also showed reduced activity. Specific miRNAs (tae-miR169, tae-miR-399, tae-miR-395) may contribute to male sterility, with impaired sucrose decomposition and increased starch degradation leading to deficient pollen starch accumulation.</p>

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Comprehensive analysis of miRNAs and their regulatory networks in wheat S-type cytoplasmic male sterility

  • 期刊论文

  • 发表日期：

    2025年9月27日

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  • Jianwei Wang,
  • Weilun Wang,
  • Qingsong Ba,
  • Lanlan Zhang,
  • Gensheng Zhang,
  • Weixue Shu,
  • Han Yang,
  • Guiping Li

摘要

Cytoplasmic male sterility (CMS) lines are crucial for exploiting wheat heterosis and producing hybrid seeds. MicroRNAs (miRNAs), key endogenous non-coding small RNAs in plant growth and development, are understudied in regulating wheat male sterility. This study employed comparative small RNA sequencing to analyze the anther of the CMS line “S1376A” and its maintainer line “1376B” using high-throughput sequencing. A total of 114 known miRNAs belonging to 2473 conserved and 364 non-conserved miRNA families were isolated, and 2837 potential novel miRNA families were identified for the first time in floral buds of wheat. Multiple miRNA target genes associated with CMS occurrence encode essential transcription factors and proteins, likely involved in biological processes like starch and sucrose metabolism regulation, MYB expression, glycolysis, and microtubule formation. In “S1376A,” sucrose synthase and phosphate synthase activities were lower than in “1376B”; starch metabolism enzymes (α-amylase, β-amylase, etc.) also showed reduced activity. Specific miRNAs (tae-miR169, tae-miR-399, tae-miR-395) may contribute to male sterility, with impaired sucrose decomposition and increased starch degradation leading to deficient pollen starch accumulation.