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<p>Bacterial blight caused by the bacterial pathogen <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> (<i>Xoo</i>) limits rice yield in all major rice-growing regions of the world, especially in irrigated lowland and rain-fed conditions where predisposition factors favor disease development to epidemic proportions. Developing new high-yielding rice varieties resistant to bacterial leaf blight (BB) is an effective strategy for controlling BB. This investigation was conducted to identify and establish the bacterial leaf blight-resistant DHs from 200 doubled haploid population developed from an elite <i>indica</i> rice hybrid, BS6444G of rice through the employment of molecular markers associated with bacterial blight-resistant genes (<i>Xa4</i>, <i>xa5</i>, <i>Xa7</i>, <i>Xa21</i>). Based on disease scoring, 200 DHs were categorized into 12 highly resistant, 24 resistant, 42 moderately resistant, and 21 moderately susceptible while 64 and 38 were found susceptible and highly susceptible, respectively. The most frequent BB gene resistant combination is (<i>Xa4</i>+<i>xa5</i>+<i>Xa7</i>+<i>Xa21</i>) &gt; (<i>Xa4</i>+<i>Xa7</i>+<i>Xa21</i>) &gt; (<i>xa5</i>+<i>Xa4</i>+<i>Xa7</i>) &gt; (<i>xa5</i>+<i>Xa7</i>+<i>Xa21</i>). The polymorphism information content (PIC) value ranged from 0.224 in the case of <i>Xa21</i> to 0.372 for <i>xa5</i> with a mean PIC of 0.319. Analysis of molecular variance (AMOVA) divulged increased genetic distinction among DHs than within DHs. Principal coordinate analysis (PCoA) exhibited 76.14% of the total variation among DHs. The resistant DHs distinguished in the current study can be utilized instantaneously as resistant donors in rice breeding.</p>

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Efficiency of doubled haploid technology in mining of multiple BB resistance genes from indica rice hybrid

  • 期刊论文

  • 发表日期：

    2025年4月30日

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  • Sudhansu Sekhar Bhuyan,
  • Byomkesh Dash,
  • Prachitara Rout,
  • Nupur Naik,
  • Manjusha Chandravani,
  • Nibedita Swain,
  • Ram Lakhan Verma,
  • Jawahar Lal Katara,
  • Arup Kumar Mukherjee,
  • C. Parameswaran,
  • BN. Devanna,
  • Snigdha Samir Pattnaik,
  • Sanghamitra Samantaray

摘要

Bacterial blight caused by the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo) limits rice yield in all major rice-growing regions of the world, especially in irrigated lowland and rain-fed conditions where predisposition factors favor disease development to epidemic proportions. Developing new high-yielding rice varieties resistant to bacterial leaf blight (BB) is an effective strategy for controlling BB. This investigation was conducted to identify and establish the bacterial leaf blight-resistant DHs from 200 doubled haploid population developed from an elite indica rice hybrid, BS6444G of rice through the employment of molecular markers associated with bacterial blight-resistant genes (Xa4, xa5, Xa7, Xa21). Based on disease scoring, 200 DHs were categorized into 12 highly resistant, 24 resistant, 42 moderately resistant, and 21 moderately susceptible while 64 and 38 were found susceptible and highly susceptible, respectively. The most frequent BB gene resistant combination is (Xa4+xa5+Xa7+Xa21) > (Xa4+Xa7+Xa21) > (xa5+Xa4+Xa7) > (xa5+Xa7+Xa21). The polymorphism information content (PIC) value ranged from 0.224 in the case of Xa21 to 0.372 for xa5 with a mean PIC of 0.319. Analysis of molecular variance (AMOVA) divulged increased genetic distinction among DHs than within DHs. Principal coordinate analysis (PCoA) exhibited 76.14% of the total variation among DHs. The resistant DHs distinguished in the current study can be utilized instantaneously as resistant donors in rice breeding.