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<p><UnorderedList Mark="Bullet"> <ItemContent> <p>Reversible microbial community restructuring observed in subarctic agricultural soils.</p> </ItemContent> <ItemContent> <p>Full-length sequencing revealed 13.7% bacterial and 5.2% fungal taxa unclassified in refDBs.</p> </ItemContent> <ItemContent> <p>Patescibacteria and Dependentiae accounted for 3.5% of soil microbiota, suggesting unique subarctic adaptations.</p> </ItemContent> <ItemContent> <p>While C and N-cycling enzymes remained stable, P and S metabolism decreased in agrosystems.</p> </ItemContent> <ItemContent> <p>Permafrost sediments contained 2.3-fold higher ARG diversity compared to modern tundra soils.</p> </ItemContent> </UnorderedList></p><p>Agricultural technologies play a significant role in shaping the landscape of our planet. Their impact will be particularly noticeable in subarctic and Arctic regions, where the consequences are likely to be the most significant. This study examines the functional properties of pristine and agricultural tundra soils (Histosols, Podzols) and ancient borehole sediments (aged 10 000 to 35 000 years). Using PacBio sequencing, we found that bacterial and fungal diversity varies by soil type and land use. Borehole samples showed bacterial diversity comparable to modern soils but significantly lower fungal diversity. Agricultural activity introduced fungal plant pathogens and reduced bacterial metabolic pathways. Hydrolase activity in tundra soils depended on nutrient availability and microbial diversity. Compared to modern soils, ancient deposits had a 2.3-fold greater diversity of antibiotic resistance genes (ARGs) and resistance mechanisms, despite lower microbial diversity. Environmental factors strongly influenced microbial and resistome diversity in modern forest-tundra soils. In contrast, ancient ARG diversity likely arose from antibiotic-producing species, which enriched ARGs while reducing microbial diversity. In summary, this study advances our understanding of structure-function relationships in cryogenic soil microbiomes, the transformative effects of agropedogenesis on microbial communities and resistomes, and provides critical baseline data for developing sustainable agricultural practices in permafrost-affected regions.</p>

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Agropedogenesis alters microbial communities and antibiotic resistomes in tundra soils: A comparison of modern and ancient cryogenic ecosystems in West Siberia

  • 期刊论文

  • 发表日期：

    2025年10月3日

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  • Alexey S. Vasilchenko,
  • Darya V. Poshvina,
  • Artyom A. Stepanov,
  • Alexander V. Balkin,
  • Diana S. Dilbaryan,
  • Aleksandr V. Iashnikov,
  • Elya Shmidt,
  • Olga Domanskaya,
  • Andrey V. Soromotin,
  • Timur Nizamutdinov,
  • Andrey V. Lisitsa,
  • Anastasia V. Teslya

摘要

Reversible microbial community restructuring observed in subarctic agricultural soils.

Full-length sequencing revealed 13.7% bacterial and 5.2% fungal taxa unclassified in refDBs.

Patescibacteria and Dependentiae accounted for 3.5% of soil microbiota, suggesting unique subarctic adaptations.

While C and N-cycling enzymes remained stable, P and S metabolism decreased in agrosystems.

Permafrost sediments contained 2.3-fold higher ARG diversity compared to modern tundra soils.

Agricultural technologies play a significant role in shaping the landscape of our planet. Their impact will be particularly noticeable in subarctic and Arctic regions, where the consequences are likely to be the most significant. This study examines the functional properties of pristine and agricultural tundra soils (Histosols, Podzols) and ancient borehole sediments (aged 10 000 to 35 000 years). Using PacBio sequencing, we found that bacterial and fungal diversity varies by soil type and land use. Borehole samples showed bacterial diversity comparable to modern soils but significantly lower fungal diversity. Agricultural activity introduced fungal plant pathogens and reduced bacterial metabolic pathways. Hydrolase activity in tundra soils depended on nutrient availability and microbial diversity. Compared to modern soils, ancient deposits had a 2.3-fold greater diversity of antibiotic resistance genes (ARGs) and resistance mechanisms, despite lower microbial diversity. Environmental factors strongly influenced microbial and resistome diversity in modern forest-tundra soils. In contrast, ancient ARG diversity likely arose from antibiotic-producing species, which enriched ARGs while reducing microbial diversity. In summary, this study advances our understanding of structure-function relationships in cryogenic soil microbiomes, the transformative effects of agropedogenesis on microbial communities and resistomes, and provides critical baseline data for developing sustainable agricultural practices in permafrost-affected regions.