<UnorderedList Mark="Bullet"> <ItemContent> 15 years cattle manure addition enhanced soil P availability and lettuce yield. </ItemContent> <ItemContent> gcd and phoD gene abundances increased under manure treatments, correlating with available P. </ItemContent> <ItemContent> Combined manure and chemical fertilizers led to increased microbial community diversity. </ItemContent> <ItemContent> Positive interactions within microbial networks were higher in manure-applied soils. </ItemContent> <ItemContent> Key microbial taxa were identified as crucial drivers of P transformation. </ItemContent> </UnorderedList>Long-term cattle manure application significantly influences soil phosphorus (P) cycling and associated microbial communities in agricultural systems. However, the mechanisms by which P-transforming microbial communities and their ecological networks mediate P cycling and crop productivity under sustained organic amendment remain poorly understood. This study investigated the effects of 15-year cattle manure application on soil P forms, P-solubilizing microbial communities, and lettuce (Lactuca sativa) yields across three treatments: no fertilization (control), manure-only (M), and combined manure and chemical fertilizer (M+CF). The M+CF treatment significantly enhanced lettuce yields by 77% compared to control and 41% compared to M treatment, while increasing P content by 3.9% and 2.1%, respectively. Metagenomic analysis revealed that manure application increased the diversity (Shannon index: +32.5%) and abundance (+260%) of phoD-harboring bacteria in the M treatment, while M+CF enhanced both diversity (+45.3%) and abundance (+290%) of gcd-harboring bacteria. Proteobacteria (54.2%–68.8%), Acidobacteria (24.2%–33.2%), and Gemmatimonadetes dominated the P-solubilizing bacterial communities across treatments. Network analysis demonstrated that M+CF treatment increased positive microbial correlations by 74.6% compared to control, with enhanced connectivity among keystone taxa, particularly for gcd-harboring microorganisms. Soil enzyme activities showed strong correlations with gene abundances (R2 = 0.92 for gcd-ACP; R2 = 0.86 for phoD-ALP), suggesting functional linkages between microbial community composition and P transformation processes. Overall, these findings demonstrate that appropriate long-term fertilization strategies can optimize soil P use efficiency, enhance microbial-mediated P transformations, and improve vegetable yields, providing insights for sustainable nutrient management in intensive cropping systems.

Fifteen-year cattle manure application reshapes phoD- and gcd-harboring microbiomes, enhancing vegetable yields

期刊论文
发表日期：

2025年10月6日

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Yanting Mao,
Yuan Li,
Yi Zheng,
Jihui Tian,
Xiaodong Chen,
Baoyi Zhao,
Bo Fan,
Kari Ylivainio,
Arja Louhisuo,
Mari Räty,
Narasinha J. Shurpali,
Kirsi Järvenranta,
Perttu Virkajärvi,
Baokun Lei

摘要

15 years cattle manure addition enhanced soil P availability and lettuce yield.

gcd and phoD gene abundances increased under manure treatments, correlating with available P.

Combined manure and chemical fertilizers led to increased microbial community diversity.

Positive interactions within microbial networks were higher in manure-applied soils.

Key microbial taxa were identified as crucial drivers of P transformation.

Long-term cattle manure application significantly influences soil phosphorus (P) cycling and associated microbial communities in agricultural systems. However, the mechanisms by which P-transforming microbial communities and their ecological networks mediate P cycling and crop productivity under sustained organic amendment remain poorly understood. This study investigated the effects of 15-year cattle manure application on soil P forms, P-solubilizing microbial communities, and lettuce (Lactuca sativa) yields across three treatments: no fertilization (control), manure-only (M), and combined manure and chemical fertilizer (M+CF). The M+CF treatment significantly enhanced lettuce yields by 77% compared to control and 41% compared to M treatment, while increasing P content by 3.9% and 2.1%, respectively. Metagenomic analysis revealed that manure application increased the diversity (Shannon index: +32.5%) and abundance (+260%) of phoD-harboring bacteria in the M treatment, while M+CF enhanced both diversity (+45.3%) and abundance (+290%) of gcd-harboring bacteria. Proteobacteria (54.2%–68.8%), Acidobacteria (24.2%–33.2%), and Gemmatimonadetes dominated the P-solubilizing bacterial communities across treatments. Network analysis demonstrated that M+CF treatment increased positive microbial correlations by 74.6% compared to control, with enhanced connectivity among keystone taxa, particularly for gcd-harboring microorganisms. Soil enzyme activities showed strong correlations with gene abundances (R² = 0.92 for gcd-ACP; R² = 0.86 for phoD-ALP), suggesting functional linkages between microbial community composition and P transformation processes. Overall, these findings demonstrate that appropriate long-term fertilization strategies can optimize soil P use efficiency, enhance microbial-mediated P transformations, and improve vegetable yields, providing insights for sustainable nutrient management in intensive cropping systems.