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<p><i>Mycoplasma</i> spp infections in small livestock are recognized worldwide as a significant cause of economic loss and welfare concerns in the agricultural sector. The aim of the current study was to investigate of the <i>Mycoplasma</i> genus molecular detection using PCR. In this study, 120 milk samples and 50 blood samples were obtained from sheep in the villages of Salmas County and transported on ice to the Microbiology Laboratory of the Faculty of Veterinary Medicine, Urmia University. DNA extraction was performed directly on the samples using a specific bacterial DNA extraction kit according to the manufacturer’s instructions. The <i>16&#xa0;S rRNA</i> gene was used to confirm the <i>Mycoplasma</i> genus.Evaluation of the <i>vspA</i> virulence gene was performed on milk and blood samples. Molecular results revealed that out of 120 milk samples, 23 samples (19.16%) were positive for the <i>16&#xa0;S rRNA</i> gene, and No blood samples were positive for <i>Mycoplasma</i> spp. Among the positive samples, 15 samples (12.5%) tested positive for the <i>vspA</i> virulence gene. The incidence of mastitis caused by <i>Mycoplasma agalactiae</i> can be attributed to poor hygiene during milking and sanitary deficiencies in sheep housing, which are considered main causes in the spread of mastitis.</p>

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Molecular detection of Mycoplasma spp. based on 16S rRNA and vspA genes in sheep milk and blood samples in Salmas, Iran

  • 期刊论文

  • 发表日期：

    2026年7月3日

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  • Javid Taghinejad,
  • Abdolghaffar Ownagh,
  • Ahmad Enferadi

摘要

Mycoplasma spp infections in small livestock are recognized worldwide as a significant cause of economic loss and welfare concerns in the agricultural sector. The aim of the current study was to investigate of the Mycoplasma genus molecular detection using PCR. In this study, 120 milk samples and 50 blood samples were obtained from sheep in the villages of Salmas County and transported on ice to the Microbiology Laboratory of the Faculty of Veterinary Medicine, Urmia University. DNA extraction was performed directly on the samples using a specific bacterial DNA extraction kit according to the manufacturer’s instructions. The 16 S rRNA gene was used to confirm the Mycoplasma genus.Evaluation of the vspA virulence gene was performed on milk and blood samples. Molecular results revealed that out of 120 milk samples, 23 samples (19.16%) were positive for the 16 S rRNA gene, and No blood samples were positive for Mycoplasma spp. Among the positive samples, 15 samples (12.5%) tested positive for the vspA virulence gene. The incidence of mastitis caused by Mycoplasma agalactiae can be attributed to poor hygiene during milking and sanitary deficiencies in sheep housing, which are considered main causes in the spread of mastitis.