Scholarlink

<p>This study aimed to evaluate the effect of different sanitization procedures on the eggshell surface and yolk sac microbiota of one-day-old chicks. Hatching eggs were collected from a 70-wk-old Cobb500<sup>®</sup> commercial broiler breeder flock and distributed in a completely randomized design with seven treatments: fumigation with paraformaldehyde (4.63&#xa0;g/m<sup>3</sup>/30 min; FORM), fumigation with ozone (5-15ppm/30 min; Ozone), ultraviolet light-C irradiation (8.09 mW/cm<sup>2</sup>; 120&#xa0;s; UV-C), hydrogen peroxide spraying (3%; 0.69 mL/egg; H<sub>2</sub>O<sub>2</sub>), peracetic acid spraying (0.3%; 0.69mL/egg; PAC), water spraying (0.69mL/egg; Water) and without sanitization (dry control - Dry). Culture-dependent methodology was carried out to isolate the microorganisms from eggshell surface (<i>n</i> = 224) and yolk sac in one-day-old chicks (<i>n</i> = 91), followed by identification by mass spectrometry (MALDI-TOF). Proteobacteria phylum was dominant on egg structures evaluated, with nineteen genera identified on the eggshell surface and twelve in the yolk sac. The most abundant genera found on eggshell surfaces and in yolk sacs were <i>Staphylococcus</i> spp. (18%) and <i>Escherichia</i> spp. (32.6%), respectively. Among the sanitization protocols tested, ultraviolet light-C irradiation and peracetic acid spraying decreased the presence of <i>Staphylococcus</i> spp. and <i>Escherichia</i> spp. in hatching eggs. Eggshell and yolk sac microbiota of newly hatched chicks were dominated by <i>Staphylococcus</i> and <i>Escherichia</i> species, respectively. UV-C irradiation and peracetic acid effectively reduced eggshell <i>Staphylococcus</i>, with peracetic acid also decreasing yolk sac <i>Escherichia</i>. These findings support UV-C and peracetic acid as effective alternatives to formaldehyde in commercial hatcheries.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Alternative protocols for sanitizing hatching eggs and their effects on the microbiota of eggshell surface and chick yolk sac

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年3月6日

查看全文
  • Erica Faria Melo,
  • Winnie Luiza Santos Clímaco,
  • Mariana Andre Pompeu,
  • Mariana Cristina Vieira,
  • Henrique César Pereira Figueiredo,
  • Marcelo Resende de Souza,
  • Itallo Conrado Sousa Araujo,
  • Leonardo Jose Camargos Lara

摘要

This study aimed to evaluate the effect of different sanitization procedures on the eggshell surface and yolk sac microbiota of one-day-old chicks. Hatching eggs were collected from a 70-wk-old Cobb500® commercial broiler breeder flock and distributed in a completely randomized design with seven treatments: fumigation with paraformaldehyde (4.63 g/m3/30 min; FORM), fumigation with ozone (5-15ppm/30 min; Ozone), ultraviolet light-C irradiation (8.09 mW/cm2; 120 s; UV-C), hydrogen peroxide spraying (3%; 0.69 mL/egg; H2O2), peracetic acid spraying (0.3%; 0.69mL/egg; PAC), water spraying (0.69mL/egg; Water) and without sanitization (dry control - Dry). Culture-dependent methodology was carried out to isolate the microorganisms from eggshell surface (n = 224) and yolk sac in one-day-old chicks (n = 91), followed by identification by mass spectrometry (MALDI-TOF). Proteobacteria phylum was dominant on egg structures evaluated, with nineteen genera identified on the eggshell surface and twelve in the yolk sac. The most abundant genera found on eggshell surfaces and in yolk sacs were Staphylococcus spp. (18%) and Escherichia spp. (32.6%), respectively. Among the sanitization protocols tested, ultraviolet light-C irradiation and peracetic acid spraying decreased the presence of Staphylococcus spp. and Escherichia spp. in hatching eggs. Eggshell and yolk sac microbiota of newly hatched chicks were dominated by Staphylococcus and Escherichia species, respectively. UV-C irradiation and peracetic acid effectively reduced eggshell Staphylococcus, with peracetic acid also decreasing yolk sac Escherichia. These findings support UV-C and peracetic acid as effective alternatives to formaldehyde in commercial hatcheries.