Scholarlink

<p>Pertussis, a vaccine-preventable respiratory disease caused by <i>Bordetella pertussis</i>, has resurged in Brazil despite sustained whole-cell (wP) vaccine coverage. In 2024, the country experienced its largest outbreak since 2014, with Espírito Santo reporting a 91% increase in cases between 2024 and mid-2025. This study aimed to characterize the allelic profiles of <i>B. pertussis</i> isolates from the 2024 Espírito Santo outbreak and to compare them with the Bp137 wP vaccine strain. Five isolates were sequenced using the Illumina Respiratory Pathogen ID/AMR Enrichment Panel Kit. Seven virulence-associated genes, including the <i>ptx</i> locus (five subunits of the pertussis toxin) and the <i>ptxP</i> promoter region, were analyzed through comparative genomic approaches. All isolates shared an identical allelic profile, suggesting the circulation of a single clonal lineage. Divergences from Bp137 were identified in <i>fim2</i>, <i>ptxP</i>, <i>ptxA</i>, and <i>ptxC</i>, loci associated with adhesion, toxin production, and immune evasion, possibly reflecting vaccine-driven diversification. These findings highlight potential antigenic mismatch and the impact of reduced population immunity in the post-COVID-19 period. Despite the limited sample size, this study underscores the scarcity of allele profiling in Brazil and the need for expanded molecular surveillance to support pertussis control strategies.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Molecular surveillance of Bordetella pertussis during the 2024 outbreak in Southeast Brazil: allelic profiles and comparison to vaccine strain

  • 期刊论文

  • 发表日期：

    2026年3月24日

查看全文
  • Thiago J. Sousa,
  • Felipe D. Gatti,
  • Joana Z. Nodari,
  • Gabriela C. de Mendonça,
  • Anna Clara G. Có,
  • Thabata C. D. Damasceno,
  • Nayara C. Silva,
  • Lílian S. Lavagnoli,
  • Danielle G. P. Lyra,
  • Jaqueline P. Goulart,
  • Rodrigo Ribeiro-Rodrigues

摘要

Pertussis, a vaccine-preventable respiratory disease caused by Bordetella pertussis, has resurged in Brazil despite sustained whole-cell (wP) vaccine coverage. In 2024, the country experienced its largest outbreak since 2014, with Espírito Santo reporting a 91% increase in cases between 2024 and mid-2025. This study aimed to characterize the allelic profiles of B. pertussis isolates from the 2024 Espírito Santo outbreak and to compare them with the Bp137 wP vaccine strain. Five isolates were sequenced using the Illumina Respiratory Pathogen ID/AMR Enrichment Panel Kit. Seven virulence-associated genes, including the ptx locus (five subunits of the pertussis toxin) and the ptxP promoter region, were analyzed through comparative genomic approaches. All isolates shared an identical allelic profile, suggesting the circulation of a single clonal lineage. Divergences from Bp137 were identified in fim2, ptxP, ptxA, and ptxC, loci associated with adhesion, toxin production, and immune evasion, possibly reflecting vaccine-driven diversification. These findings highlight potential antigenic mismatch and the impact of reduced population immunity in the post-COVID-19 period. Despite the limited sample size, this study underscores the scarcity of allele profiling in Brazil and the need for expanded molecular surveillance to support pertussis control strategies.