Objective <p>The primary objective of this study was to assess the prevalence of <i>Bordetella bronchiseptica</i> infection in dogs of West Azerbaijan province, Iran through molecular techniques.</p> Methods <p>A total of 100 blood samples from dogs were collected, and information regarding their age, sex, breed, housing conditions, and Presence or absence of respiratory diseases signs was recorded. The identification of positive samples was accomplished through PCR and sequencing, with subsequent analysis of the gene sequences conducted using Oligo7 software.</p> Results <p>The results revealed that 17 of the blood samples tested positive for <i>B. bronchiseptica</i>. The highest rate of infection was recorded in brachycephalic dogs, at 41.17%, whereas the lowest rate was found in non-brachycephalic dogs, at 4.54%. Data analysis indicated that age, sex, and housing conditions did not have a significant association with <i>B. bronchiseptica</i> infection. However, the presence or absence of respiratory disease signs demonstrated a significant correlation with infection status. This study highlights a notable prevalence of <i>B. bronchiseptica</i> infection among dogs, suggesting that both kenneled and pet dogs may present a risk factor for human infections with <i>B. bronchiseptica</i>.</p>