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<p><i>Escherichia coli</i> can contaminate the marine environment through sewage and accumulate in bivalve molluscs. We assessed antimicrobial resistance (AMR) mechanisms and genetic diversity of 69 <i>E. coli</i> isolates recovered from <i>in natura</i>, boiled, and purchased <i>Perna perna</i> mussels in Niterói city, Brazil. All isolates were sensitive to cefoxitin, imipenem, meropenem, and fosfomycin. In contrast, 61 (88.4%) isolates were not susceptible to streptomycin. We found ten (14.5%) multidrug-resistant (MDR) isolates. Extended-spectrum beta-lactamase (ESBL) production was detected in 30 (43.5%) isolates, with 14 (46.7%) isolates carrying ESBL genes (<i>bla</i><sub><i>TEM</i></sub>, <i>bla</i><sub><i>CTX−M9</i></sub>, <i>bla</i><sub><i>SHV</i></sub>, <i>bla</i><sub><i>CTX−M2</i></sub>, and <i>bla</i><sub><i>CTX−M8/25</i></sub>). Quinolone/fluoroquinolone resistance was observed in 12 (17.4%) isolates, with <i>qnrS</i> and <i>qnrB</i> genes identified. Nine (13%) isolates were resistant to folate pathway inhibitors, with <i>sul1</i> and <i>sul2</i> genes identified. Only one (1.5%) isolate had the <i>intI</i> integrase gene. Most isolates had the A/C replicon (73.9%) and belonged to phylogenetic group B1 (50.7%). PFGE analysis revealed the existence of 54 genotypes, with four clusters containing isolates from different sources. The presence of ESBL-producing strains, AMR genes, and diverse plasmid replicons highlights the role of marine environments in AMR dissemination. Continuous AMR surveillance in marine ecosystems is crucial to mitigate public health risks linked to seafood consumption.</p>

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Antimicrobial resistance and genetic diversity of Escherichia coli isolated from marine bivalves

  • 期刊论文

  • 发表日期：

    2025年5月22日

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  • Jailton Lobo da Costa Lima,
  • Marcelly Miranda Aybal Jayme,
  • Felipe Piedade Gonçalves Neves,
  • Angela Corrêa de Freitas-Almeida,
  • Mara Lúcia Penna Queiroz,
  • Fabio Vieira de Araujo

摘要

Escherichia coli can contaminate the marine environment through sewage and accumulate in bivalve molluscs. We assessed antimicrobial resistance (AMR) mechanisms and genetic diversity of 69 E. coli isolates recovered from in natura, boiled, and purchased Perna perna mussels in Niterói city, Brazil. All isolates were sensitive to cefoxitin, imipenem, meropenem, and fosfomycin. In contrast, 61 (88.4%) isolates were not susceptible to streptomycin. We found ten (14.5%) multidrug-resistant (MDR) isolates. Extended-spectrum beta-lactamase (ESBL) production was detected in 30 (43.5%) isolates, with 14 (46.7%) isolates carrying ESBL genes (blaTEM, blaCTX−M9, blaSHV, blaCTX−M2, and blaCTX−M8/25). Quinolone/fluoroquinolone resistance was observed in 12 (17.4%) isolates, with qnrS and qnrB genes identified. Nine (13%) isolates were resistant to folate pathway inhibitors, with sul1 and sul2 genes identified. Only one (1.5%) isolate had the intI integrase gene. Most isolates had the A/C replicon (73.9%) and belonged to phylogenetic group B1 (50.7%). PFGE analysis revealed the existence of 54 genotypes, with four clusters containing isolates from different sources. The presence of ESBL-producing strains, AMR genes, and diverse plasmid replicons highlights the role of marine environments in AMR dissemination. Continuous AMR surveillance in marine ecosystems is crucial to mitigate public health risks linked to seafood consumption.