<p>Inspired by the efficient signal transmission in neurons, nanofluidic systems have been developed to emulate memristive or gating effects, showing promising prospects for neuromorphic computing and bioabiotic interfaces. However, the existing devices have failed to integrate these two fundamental neuronal properties within a single device; thus, falling short of simulating the complex and integrated signal transmission and processing of biological neural systems. Here, we demonstrate a nanofluidic memristor (DAb-NM) based on the graphene oxide fibers for neuromorphic neurotransmitter signal transmission. The device features confined nanochannel with its dimension matching with that of transmitted neurotransmitters, inducing intense electrostatic repulsion and concentration polarization. The high interfacial energy barrier for dopamine (DA) ion transport induces hysteretic ion transport that exhibits a memory effect of paired-pulse facilitation (PPF), and a gating effect which necessitates charge accumulation to open the channel. By integrating memristive and gating effects within a single device, the nanofluidic device not only realizes Pavlovian learning capabilities but also enables neuromorphic information processing when interfacing with living systems. This study provides a novel strategy for integrating complex neurotransmitter-based neural features, advancing the development of intelligent biohybrid interfaces.</p> Graphical Abstract <p></p>