<p>What is neutral affect? And how can we elicit it? Based on a targeted review of the literature, the present study provides a large-scale, pre-registered investigation of affective responses to 39 neutral film clips. Participants (<i>N</i> = 996) watched random subsets of eight neutral and (for manipulation checks) one amusement- and one sadness-eliciting film clip and reported on their affective experiences (i.e., 16 discrete emotions, valence, arousal) at baseline and after each film clip. To probe neutral affect, we examined discrete affect intensity, variability, and peakedness (following discrete emotion models) and valence and arousal (following dimensional emotion models). Results showed that participants experienced relatively high levels of positive affect at baseline. We conducted eight analyses (six using affective response indices derived from discrete and dimensional models and two assessing peakedness) to identify the most neutral films (i.e., those eliciting the lowest levels of affective responses, or for peakedness analyses, the least differentiation among discrete emotions). Five neutral film clips elicited the lowest levels of affective responses across all eight analyses, with one clip eliciting the lowest levels of affect across four indices and an overall affect composite. Four to 32 other neutral film clips elicited significantly greater affective responses than the film that elicited the lowest level of each of the affective response indices. Follow-up analyses probed generalizability across age and gender. Findings from this first large-scale investigation of neutral film clips provide a robust foundation for future studies with neutral film clips and suggest directions for future research.</p>