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Purpose <p>2-methyl-4-chlorophenoxyacetic acid (MCPA) is a commonly used herbicide in agriculture. Beneficial microorganisms can extensively improve soil quality and degrade pesticides to promote plant growth. However, the performance of bacteria surviving in the presence of MCPA and whether differential gene expression occurs in the target strain under MCPA stress remain unknown.</p> Methods <p>In this study, MCPA-degrading bacteria were identified. Genome and transcriptome sequencing analyses were performed to identify the target genes and metabolic processes in obtained bacteria.</p> Results <p>A total of nine strains were obtained with the ability to grow under MCPA stress. The main metabolite pathways of bacteria were involved in glycan biosynthesis and metabolism, carbohydrate metabolism, amino acid metabolism, and membrane transport. In strain MAN3, genes including C4003893, C4006502, and C4001699 were upregulated. Our study indicates that bacteria considerably adopt different metabolite pathways to cope with MCPA.</p> Conclusions <p>The results provide new insight into the isolation of MCPA-degrading bacteria and expand the knowledge base to gene expression in bacteria coping with MCPA toxicity. Collectively, the findings show the mechanisms by which bacteria enable soil improvement under MCPA contamination.</p>

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Transcriptome Sequencing Analysis of 2-Methyl-4-Chlorophenoxyacetic acid-Degrading Bacteria and their Applications in Reducing Toxicity to Cucurbit Seedlings

  • 期刊论文

  • 发表日期：

    2026年2月10日

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  • Jian Zhang,
  • Zhao Xie,
  • Tongchun Gao,
  • Pengcheng Wang

摘要

Purpose

2-methyl-4-chlorophenoxyacetic acid (MCPA) is a commonly used herbicide in agriculture. Beneficial microorganisms can extensively improve soil quality and degrade pesticides to promote plant growth. However, the performance of bacteria surviving in the presence of MCPA and whether differential gene expression occurs in the target strain under MCPA stress remain unknown.

Methods

In this study, MCPA-degrading bacteria were identified. Genome and transcriptome sequencing analyses were performed to identify the target genes and metabolic processes in obtained bacteria.

Results

A total of nine strains were obtained with the ability to grow under MCPA stress. The main metabolite pathways of bacteria were involved in glycan biosynthesis and metabolism, carbohydrate metabolism, amino acid metabolism, and membrane transport. In strain MAN3, genes including C4003893, C4006502, and C4001699 were upregulated. Our study indicates that bacteria considerably adopt different metabolite pathways to cope with MCPA.

Conclusions

The results provide new insight into the isolation of MCPA-degrading bacteria and expand the knowledge base to gene expression in bacteria coping with MCPA toxicity. Collectively, the findings show the mechanisms by which bacteria enable soil improvement under MCPA contamination.