<p>Purpose: Root pressure is crucial for long-distance water transport in the xylem of plants and is regulated by root physiological activities and environmental factors. However, how nutrient elements affect root pressure remains unclear. <i>Dracaena sanderiana</i> is an ideal model for studying root pressure regulation owing to its good adaptation to hydroponic conditions and consistent positive root pressure. Herein, the effects of different types and concentrations of nutrient elements on the root pressure of hydroponic <i>D. sanderiana</i> were investigated. Methods: Hydroponic <i>D. sanderiana</i> root pressure was continuously monitored using pressure sensors under various treatments: (1) acidic mineral nutrient solutions (pH 4.5 H₃BO₃ (320 <i>mM</i>),10 and 20 <i>mM</i> KH₂PO₄); (2) alkaline mineral nutrient solution (pH 9 KOH); (3) neutral mineral nutrient solutions (10 and 20 <i>mM</i> KCl, KNO₃, and Ca(NO₃)₂); (4) 1/8, 1/4, and full strength Hoagland nutrient solution (HNS); and (5) amino acid solutions (5, 10 and 20 <i>mM</i> glutamic acid, lysine, and leucine). Results: Both H₃BO₃ and KH₂PO₄ reduced the root pressure, whereas the root pressure increased slightly with KOH, Ca(NO₃)₂, or low concentrations of KCl and KNO₃; meanwhile, high concentrations of KCl, KNO₃, and HNS lowered the root pressure. Whereas the 10 and 20 <i>mM</i> amino acid treatments reduced root pressure in a clear concentration-dependent manner, the 5 <i>mM</i> treatments produced three distinct responses depending on the amino acid supplied. Conclusions:&#xa0;<i>D. sanderiana</i> root pressure is sensitive to nutrient composition and can be used for assessing the effects of nutrients on plants. This study contributes to optimizing <i>D. sanderiana</i> cultivation and provides a new perspective for researching the mechanisms of root pressure generation.</p>