<p>Litter serves as a critical factor influencing the formation, stabilization, and turnover of soil organic carbon (SOC) in forest soils, and it is also a significant factor in the turnover of soil aggregates.However, the influence of karst forest litter on SOC through aggregate turnover remains unclear. This study simulated the input regulation of surface litter by in-situ replacement of C₃/C₄ soil columns in the field.Three litter addition treatment were established, no litter (CK), single litter (X1,L1,R1), and double litter (X2,L2,R2), to analyze changes in aggregate composition and stability and the SOC and component contents after 6 and 12 months of input with three different types of litter:<i>Toona sinensis</i>, <i>Koelreuteria paniculata</i>, and <i>Zenia insignis</i> Chun. Results showed that the input of litter markedly increased the amount of large macroaggregates (LMA), the average weight diameter (MWD), and the geometric average diameter (MGD), reduced the amount of small macroaggregates (SMA) and microaggregates (MI); the input duration of litter markedly affected the accumulation of SOC and aggregate organic carbon (OC) (<i>P</i> &lt; 0.001), increased the particulate organic carbon (POC) and mineral-associated organic carbon (MAOC) contents in the entire soil; The contribution rate of OC from different particle size aggregates to total SOC is as follows: SMA-OC contribution rate [<i>F</i> (SMA-OC)] &gt; LMA-OC contribution rate [<i>F</i> (LMA-OC)] &gt; MI-OC contribution rate [<i>F</i> (MI-OC)]; Structural equation modeling indicates that the field input incubation timehad the greatest overall impact on SOC by regulating aggregate OC. We concluded that litter input facilitates the transfer of smaller aggregates to larger aggregates, enhances aggregate structure and stability; and promotes the allocation of SOC from smaller aggregates to larger aggregates, thereby enhancing the accumulation of SOC.</p>