Effects of Long-Term Nitrogen Addition on Glomalin-Related Soil Protein Dynamics, Soil Aggregate Stability and Organic Carbon Sequestration in the Boreal Forest
摘要
We conducted a field nitrogen (N) addition experiment in boreal forests to clarify how Glomalin-related soil protein (GRSP), soil aggregates, and soil organic carbon (SOC) stocks interact and how these relationships vary seasonally under N deposition. In order to measure GRSP, SOC, aggregate size distribution, and SOC in aggregates across spring, summer, and autumn, the field experiments with different N addition levels (the control, LN, MN, HN; 0, 25, 50, and 75 kg N ha⁻¹ year⁻¹) were conducted in a boreal forest. LN and MN treatments enhanced easily extractable GRSP (EE-GRSP) production in summer and autumn. Meanwhile, MN treatment increased total GRSP (T-GRSP) accumulation in summer, whereas HN treatment significantly reduced T-GRSP content in spring and autumn, and also suppressed arbuscular mycorrhizal fungi activity in autumn. Additionally, MN and HN treatments resulted in significantly higher GRSP content in summer than in spring and autumn. LN and MN treatments in spring and summer significantly increased the SOC content and proportion of > 2 mm size soil macroaggregates, while significantly increasing mean weight diameter (MWD) and geometric mean diameter (GMD). EE-GRSP and T-GRSP were highly positively linked with SOC, while T-GRSP also demonstrated substantial positive correlation with MWD and GMD. Our results further demonstrated that MN treatment considerably raised the SOC content in > 2 mm aggregates in summer, while LN and MN treatments significantly enhanced the contribution of SOC in > 2 mm aggregates to bulk SOC.