Scholarlink

<p><i>Brachiaria</i> grasses dominate Brazilian pastures, yet studies on their association with diazotrophic bacteria are limited. Here, we examined the growth-promoting effects of eight bacterial strains per <i>Brachiaria</i> genotype (eleven genotypes in total), selected from a working culture collection of 32 strains, in a soil box experiment. Subsequently, five strains were evaluated under field conditions with three <i>B. brizantha</i> genotypes (Marandu, Paiaguás and Xaraés) to identify potential biofertilizer candidates. Methods: Both experiments were conducted using a completely randomized block design, with three and four replications, respectively. In the greenhouse, each <i>Brachiaria</i> genotype, analysed independently, consisted of 10 treatments: an uninoculated control, nitrogen fertilizer, and eight bacterial strains while the field experiment included eight treatments: five strains (greenhouse study), a commercial inoculant, nitrogen fertilizer, and an uninoculated control. In the greenhouse experiment, shoots were harvested at 2, 4, and 6&#xa0;months after cutting and subsequently regrowth. In the field experiment, plants were harvested at 4, 7, 10, and 13&#xa0;months under the same growth cycle. Total dry biomass and nutrient uptake (nitrogen, phosphorus, and potassium) were analysed for each harvest in both experiments. The box experiment revealed that inoculated <i>Brachiaria</i> plants exhibited higher biomass production and NPK nutrient uptake compared to the absolute control. Notable increases included up to 23% in dry biomass, 37% in total N, 24% in total K, and 32% in total P, varying by strain and genotype. In the field, the Paiaguás genotype showed a significant response to <i>Azospirillum argentinense</i> strains NRB085 and NRB214, with statistically superior increases in dry biomass and NPK uptake compared to the control. The findings indicate that strains NRB085 and NRB214 exhibit good biofertilizer potential, positioning these <i>A. argentinense</i> strains as promising inoculant candidates for improving <i>Brachiaria</i> pasturelands.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Novel Diazotrophic Bacterial Strains to Enhance Biomass and Nutrient Uptake in Brachiaria Genotypes

  • 期刊论文

  • 发表日期：

    2026年1月26日

查看全文
  • Nathália Virgínia da Silva Ribeiro,
  • Gabriel Martins Arruda,
  • Márcia Soares Vidal,
  • Gabriela Cavalcanti Alves,
  • Janaina Ribeiro Costa Rouws,
  • Ederson da Conceição Jesus,
  • Stefan Schwab,
  • Sanzio Carvalho Lima Barrios,
  • José Ivo Baldani

摘要

Brachiaria grasses dominate Brazilian pastures, yet studies on their association with diazotrophic bacteria are limited. Here, we examined the growth-promoting effects of eight bacterial strains per Brachiaria genotype (eleven genotypes in total), selected from a working culture collection of 32 strains, in a soil box experiment. Subsequently, five strains were evaluated under field conditions with three B. brizantha genotypes (Marandu, Paiaguás and Xaraés) to identify potential biofertilizer candidates. Methods: Both experiments were conducted using a completely randomized block design, with three and four replications, respectively. In the greenhouse, each Brachiaria genotype, analysed independently, consisted of 10 treatments: an uninoculated control, nitrogen fertilizer, and eight bacterial strains while the field experiment included eight treatments: five strains (greenhouse study), a commercial inoculant, nitrogen fertilizer, and an uninoculated control. In the greenhouse experiment, shoots were harvested at 2, 4, and 6 months after cutting and subsequently regrowth. In the field experiment, plants were harvested at 4, 7, 10, and 13 months under the same growth cycle. Total dry biomass and nutrient uptake (nitrogen, phosphorus, and potassium) were analysed for each harvest in both experiments. The box experiment revealed that inoculated Brachiaria plants exhibited higher biomass production and NPK nutrient uptake compared to the absolute control. Notable increases included up to 23% in dry biomass, 37% in total N, 24% in total K, and 32% in total P, varying by strain and genotype. In the field, the Paiaguás genotype showed a significant response to Azospirillum argentinense strains NRB085 and NRB214, with statistically superior increases in dry biomass and NPK uptake compared to the control. The findings indicate that strains NRB085 and NRB214 exhibit good biofertilizer potential, positioning these A. argentinense strains as promising inoculant candidates for improving Brachiaria pasturelands.