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<p>The alkaline mixture of waste fluid from oil and gas drilling may pose a risk to plants and soil during transportation and disposal due to its complex composition. In our experiment, four waste drilling fluid stress treatment gradients were established by mixing 0, 50, 100, and 150&#xa0;mL of waste fluid (0&#xa0;mL for the control) into the substrate. In addition to assessing soil chemical characteristics, enzyme activities, and plant physiological and biochemical markers, a comprehensive evaluation was conducted using 16S rRNA gene sequencing to analyze changes in the soil microbial community. This study demonstrates that exposure to waste fluid stress inhibited the germination and growth of <i>Trifolium repens</i>. Increasing concentrations of the waste fluid led to a progressive decline in key physiological and biochemical parameters of the plant, including photosynthetic efficiency and antioxidant enzyme activity. Microbial community analysis revealed that waste liquid stress decreased both the diversity and abundance of soil bacteria, particularly in the absence of white clover. However, planting white clover appeared to enhance the relative abundance of microbial communities and improve soil quality. Additionally, total nitrogen, total phosphorus and organic matter had significant positive effects on bacterial community diversity. This study offers valuable insights into the impact of waste drilling fluid on the growth of white clover and soil ecology, providing theoretical guidance for the ecological restoration of oil and gas sites.</p>

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Physiological, Biochemical, and Soil Microbial Community Responses of Trifolium repens L. to Spent Drilling Fluid Stress: Insights for Enhanced Vegetation Restoration

  • 期刊论文

  • 发表日期：

    2025年12月5日

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  • Lu Yang,
  • Qin Xin,
  • Yongjun Fei,
  • Yujie Yang,
  • Die Hu,
  • Zihan Cheng

摘要

The alkaline mixture of waste fluid from oil and gas drilling may pose a risk to plants and soil during transportation and disposal due to its complex composition. In our experiment, four waste drilling fluid stress treatment gradients were established by mixing 0, 50, 100, and 150 mL of waste fluid (0 mL for the control) into the substrate. In addition to assessing soil chemical characteristics, enzyme activities, and plant physiological and biochemical markers, a comprehensive evaluation was conducted using 16S rRNA gene sequencing to analyze changes in the soil microbial community. This study demonstrates that exposure to waste fluid stress inhibited the germination and growth of Trifolium repens. Increasing concentrations of the waste fluid led to a progressive decline in key physiological and biochemical parameters of the plant, including photosynthetic efficiency and antioxidant enzyme activity. Microbial community analysis revealed that waste liquid stress decreased both the diversity and abundance of soil bacteria, particularly in the absence of white clover. However, planting white clover appeared to enhance the relative abundance of microbial communities and improve soil quality. Additionally, total nitrogen, total phosphorus and organic matter had significant positive effects on bacterial community diversity. This study offers valuable insights into the impact of waste drilling fluid on the growth of white clover and soil ecology, providing theoretical guidance for the ecological restoration of oil and gas sites.