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<p>The study aimed to evaluate the protective role of jasmonic acid (JA) against mancozeb (MNZ)-induced physiological and molecular toxicity in <i>Vigna radiata</i>. Specifically, it investigated whether JA priming could alleviate oxidative stress, photosynthetic impairment, and stomatal dysfunction caused by MNZ exposure.&#xa0;<i>V. radiata</i> seeds were primed with 1 mM JA and subjected to MNZ at LC50/2 (45 mg L<sup>− 1</sup> ) and LC50 (90 mg L<sup>− 1</sup> ) concentrations. Growth parameters (germination, shoot/root length, survival) were recorded. Biochemical assays quantified enzymatic superoxide dismutase, peroxidase, catalase (SOD, POD, CAT) and non-enzymatic (proline, phenols, flavonoids) antioxidants. Photosynthetic pigment content was estimated spectrophotometrically. Gene expression of Malate dehydrogenase, Superoxide dismutase, Peroxidase and Catalase (MDH, SOD, POD, and CAT) was analyzed using RT-qPCR. Stomatal morphology was examined via scanning electron microscopy. Data were statistically analyzed using ANOVA with <i>p</i> ≤ 0.05 considered significant.&#xa0;MNZ exposure significantly reduced germination (30.7%), root length (66.7%), shoot length, photosynthetic pigments (up to 55.2%), and stomatal integrity, while inducing oxidative stress. JA priming improved these parameters, increasing germination by 14%, root length by 36.7%, and chlorophyll content by ~ 33%. Antioxidant enzymes showed notable enhancement (SOD ↑66.7%, POD ↑18.5%, CAT ↑13.3%), while non-enzymatic antioxidants also increased. JA treatment significantly upregulated stress-responsive genes (MDH ↑83.3%, SOD, POD, CAT up by 4–9%). SEM revealed partial restoration of stomatal architecture under MNZ stress.&#xa0;JA mitigates MNZ-induced toxicity by enhancing antioxidant defense systems, stabilizing photosynthetic pigments, and improving stomatal structure, thereby promoting plant resilience. These findings suggest JA as a sustainable strategy for reducing pesticide-induced stress and improving crop productivity.</p>

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Exploring the Protective Effects of Jasmonic Acid against Mancozeb-Induced Physiological and Molecular Toxicity in Vigna radiata

  • 期刊论文

  • 发表日期：

    2025年11月28日

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  • Nida Sultan,
  • Aisha Kamal

摘要

The study aimed to evaluate the protective role of jasmonic acid (JA) against mancozeb (MNZ)-induced physiological and molecular toxicity in Vigna radiata. Specifically, it investigated whether JA priming could alleviate oxidative stress, photosynthetic impairment, and stomatal dysfunction caused by MNZ exposure. V. radiata seeds were primed with 1 mM JA and subjected to MNZ at LC50/2 (45 mg L− 1 ) and LC50 (90 mg L− 1 ) concentrations. Growth parameters (germination, shoot/root length, survival) were recorded. Biochemical assays quantified enzymatic superoxide dismutase, peroxidase, catalase (SOD, POD, CAT) and non-enzymatic (proline, phenols, flavonoids) antioxidants. Photosynthetic pigment content was estimated spectrophotometrically. Gene expression of Malate dehydrogenase, Superoxide dismutase, Peroxidase and Catalase (MDH, SOD, POD, and CAT) was analyzed using RT-qPCR. Stomatal morphology was examined via scanning electron microscopy. Data were statistically analyzed using ANOVA with p ≤ 0.05 considered significant. MNZ exposure significantly reduced germination (30.7%), root length (66.7%), shoot length, photosynthetic pigments (up to 55.2%), and stomatal integrity, while inducing oxidative stress. JA priming improved these parameters, increasing germination by 14%, root length by 36.7%, and chlorophyll content by ~ 33%. Antioxidant enzymes showed notable enhancement (SOD ↑66.7%, POD ↑18.5%, CAT ↑13.3%), while non-enzymatic antioxidants also increased. JA treatment significantly upregulated stress-responsive genes (MDH ↑83.3%, SOD, POD, CAT up by 4–9%). SEM revealed partial restoration of stomatal architecture under MNZ stress. JA mitigates MNZ-induced toxicity by enhancing antioxidant defense systems, stabilizing photosynthetic pigments, and improving stomatal structure, thereby promoting plant resilience. These findings suggest JA as a sustainable strategy for reducing pesticide-induced stress and improving crop productivity.