<p>Brassinosteroids (BR) and abscisic acid (ABA) play key roles in coordinating plant responses to abiotic stress. This study aimed to comparatively analyze the hormonal and growth responses of the glycophyte <i>Glycine max</i> and the halophyte <i>Strombocarpa strombulifera</i> under different sodium salts to better understand the BR and ABA interplay. Plants were grown hydroponically and subjected to salt treatments (NaCl, Na₂SO₄ and their iso-osmotic mixture) at varying osmotic potentials. Growth parameters, water content, transpiration, and hormone accumulation (ABA, castasterone and 24-epibrassinolide) were quantified. The effects of exogenous ABA application were also evaluated. In both species, Na₂SO₄ treatments caused the most severe growth inhibition, reduced water content, and elevated ABA and BR levels. In contrast, NaCl induced moderate stress responses, while the iso-osmotic mixture of salts resulted in intermediate growth and hormonal profiles, suggesting partial mitigation of sulfate toxicity. <i>S. strombulifera</i> specifically accumulated castasterone, indicating species-specific BR biosynthesis. Exogenous ABA enhanced BR accumulation, particularly under Na₂SO₄ stress, but was insufficient to restore water balance, pointing to impaired stomatal regulation under sulfate toxicity. The presence of Na₂SO₄ disrupts hormonal homeostasis more severely than NaCl, likely through interference with sulfur metabolism and BR and ABA interplay. Their iso-osmotic mixture buffered the effects and promoted more balanced physiological responses. The species-specific hormonal patterns observed provide insights into adaptive strategies to saline environments and suggest potential targets for enhancing the tolerance. Future research should focus on identifying sulfate-sensitive components of BR and ABA interplay to inform the development of salt tolerance plants.</p>