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Purpose <p>The growth and quality of roasted tobacco are limited by the availability of potassium (K). K-solubilizing bacteria (KSB) are able to convert mineral K into a soluble form, thereby facilitating direct uptake and utilization by plants.</p> Methods <p>Here, KSB strains were isolated from tobacco rhizosphere soils in a range of districts and counties across Guizhou Province, one of the major tobacco production bases in China. High-efficiency KSB strains were obtained by comparing the solubilizing ability on K-feldspar, mica and illite, and were identified by 16S rRNA gene sequence analysis. Finally, the multifunctional characteristics (phosphorus (P) solubilization, nitrogen (N) fixation, indoleacetic acid (IAA) and siderophore productions) were investigated through qualitative and quantitative analyses.</p> Results <p>A total of 49 bacterial strains were isolated, and 11 strains exhibited enhanced K-solubilizing capacity. Furthermore, 4 strains (ALHI2, ZSB3, HB5 and FG1) were identified as having high K-solubilizing capacities for K-feldspar, mica, and illite. A reduction in pH of the culture solution was observed during the process of K solubilization, but the amount of K solubilized did not exhibit a significant correlation with the pH, suggesting that acid production is not the sole way for KSB to dissolve K-bearing minerals. Three strains of ALHI2, ZSB3 and FG1 were identified as <i>Bacillus subtilis</i>, while strain HB5 was identified as <i>Bacillus haynesii</i>. Further analyses demonstrated that all 4 strains exhibited the ability of P solubilization, N fixation, IAA and siderophore producing.</p> Conclusions <p>4 high-efficiency KSB with multifunctionality screened here can be used for the future development and preparation of bio-potassium fertilizer.</p>

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Isolation and Characterization of Efficient Multifunctional Potassium-Solubilizing Bacteria from Tobacco Rhizosphere Soil

  • 期刊论文

  • 发表日期：

    2025年8月11日

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  • Caibin Li,
  • Shuang Wang,
  • Qian Zhang,
  • Yi He,
  • Baozhen Luo,
  • Guitong Li,
  • Qimei Lin,
  • Xiaorong Zhao

摘要

Purpose

The growth and quality of roasted tobacco are limited by the availability of potassium (K). K-solubilizing bacteria (KSB) are able to convert mineral K into a soluble form, thereby facilitating direct uptake and utilization by plants.

Methods

Here, KSB strains were isolated from tobacco rhizosphere soils in a range of districts and counties across Guizhou Province, one of the major tobacco production bases in China. High-efficiency KSB strains were obtained by comparing the solubilizing ability on K-feldspar, mica and illite, and were identified by 16S rRNA gene sequence analysis. Finally, the multifunctional characteristics (phosphorus (P) solubilization, nitrogen (N) fixation, indoleacetic acid (IAA) and siderophore productions) were investigated through qualitative and quantitative analyses.

Results

A total of 49 bacterial strains were isolated, and 11 strains exhibited enhanced K-solubilizing capacity. Furthermore, 4 strains (ALHI2, ZSB3, HB5 and FG1) were identified as having high K-solubilizing capacities for K-feldspar, mica, and illite. A reduction in pH of the culture solution was observed during the process of K solubilization, but the amount of K solubilized did not exhibit a significant correlation with the pH, suggesting that acid production is not the sole way for KSB to dissolve K-bearing minerals. Three strains of ALHI2, ZSB3 and FG1 were identified as Bacillus subtilis, while strain HB5 was identified as Bacillus haynesii. Further analyses demonstrated that all 4 strains exhibited the ability of P solubilization, N fixation, IAA and siderophore producing.

Conclusions

4 high-efficiency KSB with multifunctionality screened here can be used for the future development and preparation of bio-potassium fertilizer.