<p>Roots can release 1-aminocyclopropane-1-carboxylic acid (ACC), which plays a crucial role as a signaling molecule to drive the bacterial community in the rhizosphere. In our frame of "fungal highway” studies, we hypothesized that ACC, a precursor of the plant hormone ethylene, acts as a key signaling molecule that influences the interaction between&#xa0;<i>Mucor</i> sp.&#xa0;and bacteria, facilitating bacterial dispersal through mycelial networks in the presence of varying ACC concentrations. Here, we evaluated the chemotactic effect of 1-aminocyclopropane-1-carboxylic acid (ACC) on plant-associated bacteria via the mycelium of the fungus <i>Mucor circinelloides</i> 7H. Bacterial strains were subject to chemiotaxis assays via the capillary method and bacterial dispersion systems in combination with flow cytometry. Members of the <i>Pseudomonas</i> sp. and <i>Bacillus</i> sp. 30B strains can be dispersed throughout the mycelia of <i>M. circinelloides</i> 7H. Compared with the positive control <i>Pseudomonas</i> sp. 18E (497.49 ± 16.9 cell mL⁻¹), <i>Pseudomonas</i> sp. 38E (476.7 ± 36.7 cell mL⁻¹) presented the greatest degree of cell movement. Chemotaxis assays demonstrated that ACC-degrading <i>Pseudomonas</i> strains exhibited significant movement toward ACC gradients compared with <i>Bacillus</i> sp. 30B. Flow cytometry analysis revealed that the dispersion of <i>Pseudomonas</i> sp. 38E (from 8 × 10³ to 4 × 10⁴ cells mL⁻¹) and <i>Pseudomonas</i> sp. 62F (from 5 × 10³ to 2 × 10⁴ cells mL⁻¹) increased by one order of magnitude compared with the control without ACC. This result demonstrated that the native fungal strains <i>M. circinelloides</i> 7H can disperse other genera of bacteria different from those originally found in their mycelium<i>.</i> These findings suggest that fungal mycelia use chemical signals to mediate the dispersal of beneficial bacteria in soil.</p>