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Purpose <p>Camptothecin is a precursor of many clinical anti-cancer drugs with significant activity. However, its medicinal resources are in danger due to the increasing commercial market. <i>Ophiorrhiza pumila</i>, as a folk Chinese herbal medicine, has become a model plant for studying camptothecin biosynthesis and regulation. To promote the industrial development of this resource plant, the extraction of camptothecin and plant factory of <i>O. pumila</i> were explored and optimized in this study. Methods: The process of extracting camptothecin from <i>O. pumila</i> was established and optimized using the Box-Behnken design response surface methodology. Subsequently, a plant factory system of <i>O. pumila</i> including seed germination, seedling growth, seedling acclimatization and rapid growth was built and optimized. Results: The process of extracting camptothecin from <i>O. pumila</i> was established and optimized using the Box-Behnken design response surface methodology. During the hydroponic cultivation process of <i>O. pumila</i>, the seeds harvested in the current year were selected for germination, and the seedlings were transferred to an optimized solid growth medium. After that, the seedlings were transferred to the liquid medium with the concentration of 10% and pH 5.0. Subsequently, the concentration of cultivation medium was changed to 30% after <i>O. pumila</i> seedlings entered the rapid growth stage. Finally, there was a significant increase of 3.66 times and 2.73 times in dry weight and camptothecin content in hydroponic plants compared to six-month-old soil-cultured seedlings, respectively. Conclusions: In general, this study provided a theoretical basis and new ideas for the large-scale production of camptothecin using the technology of a plant factory in <i>O. pumila</i>.</p>

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Optimizing the Extraction Process of Camptothecin and Different Stages for Plant Factory of Ophiorrhiza pumila

  • 期刊论文

  • 发表日期：

    2025年1月28日

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  • Pengyang Li,
  • Qingyan Ruan,
  • Yao Wang,
  • Chengyu Xiao,
  • Qin Zhou,
  • Yinkai Yang,
  • Yongpeng Li,
  • Liqin Li,
  • Yuxuan Sun,
  • Zelei Fang,
  • Xiaolong Hao,
  • Guoyin Kai

摘要

Purpose

Camptothecin is a precursor of many clinical anti-cancer drugs with significant activity. However, its medicinal resources are in danger due to the increasing commercial market. Ophiorrhiza pumila, as a folk Chinese herbal medicine, has become a model plant for studying camptothecin biosynthesis and regulation. To promote the industrial development of this resource plant, the extraction of camptothecin and plant factory of O. pumila were explored and optimized in this study. Methods: The process of extracting camptothecin from O. pumila was established and optimized using the Box-Behnken design response surface methodology. Subsequently, a plant factory system of O. pumila including seed germination, seedling growth, seedling acclimatization and rapid growth was built and optimized. Results: The process of extracting camptothecin from O. pumila was established and optimized using the Box-Behnken design response surface methodology. During the hydroponic cultivation process of O. pumila, the seeds harvested in the current year were selected for germination, and the seedlings were transferred to an optimized solid growth medium. After that, the seedlings were transferred to the liquid medium with the concentration of 10% and pH 5.0. Subsequently, the concentration of cultivation medium was changed to 30% after O. pumila seedlings entered the rapid growth stage. Finally, there was a significant increase of 3.66 times and 2.73 times in dry weight and camptothecin content in hydroponic plants compared to six-month-old soil-cultured seedlings, respectively. Conclusions: In general, this study provided a theoretical basis and new ideas for the large-scale production of camptothecin using the technology of a plant factory in O. pumila.