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Purpose <p>The pollution of soil with antibiotic resistance genes (ARGs) has become a significant concern. Biochar based fertilizer, as an effective soil amendment, can enhance soil fertility. However, the mechanisms underlying the effects of biochar based fertilizer on soil microbial community succession and ARGs remain unclear.</p> Methods <p>Therefore, this study investigate the effect of two levels of swine manure biochar (SMB) addition on the soil microbial community and the distribution of antibiotic resistance genes (ARGs), using high-throughput qPCR, along with 16&#xa0;S rRNA and ITS sequencing techniques.</p> Results <p>The results indicated that SMB had no significant effects on the bacterial species diversity and species uniformity, but it significantly increased the soil fungal species diversity and species uniformity, such as Basidiomycota, Chytridiomycota, Mortierellomycota, Olpidiomycota, Monoblepharomycota, and Glomeromycota. Adding SMB decreased the abundance of ARGs and mobile genetic elements (MGEs), and adding 2% SMB inhibited ARGs better than 1% SMB. Actinobacteriota, MBNT15, Bacteroidota, and Nitrospirota were identified as potential phylum level hosts of ARGs because these bacteria were significantly positively correlated with ARGs and MGEs (<i>p</i> &lt; 0.05). The correlations among the potential bacterial hosts of ARGs and fungal communities indicated that the influence of SMB on the soil fungal community structure indirectly affected the distribution of potential bacterial hosts of ARGs.</p> Conclusion <p>In general, the application of biochar based fertilizer can control the transfer and spread of ARGs. The results obtained in this study provide theoretical support for applying SMB to fertilize soil and remediate pollution by ARGs.</p>

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Biochar Based Fertilizer Reduce the Abundance of Antibiotic Resistance Genes in Rice soil

  • 期刊论文

  • 发表日期：

    2024年12月31日

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  • Ranran Zhang,
  • Chenpan Gong,
  • Yufei Zhao,
  • Yuze Gao,
  • Yifei Zhang,
  • Haifeng Zhuang,
  • Shengdao Shan

摘要

Purpose

The pollution of soil with antibiotic resistance genes (ARGs) has become a significant concern. Biochar based fertilizer, as an effective soil amendment, can enhance soil fertility. However, the mechanisms underlying the effects of biochar based fertilizer on soil microbial community succession and ARGs remain unclear.

Methods

Therefore, this study investigate the effect of two levels of swine manure biochar (SMB) addition on the soil microbial community and the distribution of antibiotic resistance genes (ARGs), using high-throughput qPCR, along with 16 S rRNA and ITS sequencing techniques.

Results

The results indicated that SMB had no significant effects on the bacterial species diversity and species uniformity, but it significantly increased the soil fungal species diversity and species uniformity, such as Basidiomycota, Chytridiomycota, Mortierellomycota, Olpidiomycota, Monoblepharomycota, and Glomeromycota. Adding SMB decreased the abundance of ARGs and mobile genetic elements (MGEs), and adding 2% SMB inhibited ARGs better than 1% SMB. Actinobacteriota, MBNT15, Bacteroidota, and Nitrospirota were identified as potential phylum level hosts of ARGs because these bacteria were significantly positively correlated with ARGs and MGEs (p < 0.05). The correlations among the potential bacterial hosts of ARGs and fungal communities indicated that the influence of SMB on the soil fungal community structure indirectly affected the distribution of potential bacterial hosts of ARGs.

Conclusion

In general, the application of biochar based fertilizer can control the transfer and spread of ARGs. The results obtained in this study provide theoretical support for applying SMB to fertilize soil and remediate pollution by ARGs.