Scholarlink

<p><i>Hyptis suaveolens</i> (L.) Poit is known for its insecticidal properties. However, the mechanism of action in bait formulations remains poorly understood. This study evaluated the insecticidal activity of <i>H. suaveolens</i> leaf-based bait (HSLB) against <i>Periplaneta americana</i> using integrated in vivo and in silico approaches. Forty-five cockroaches were randomly assigned into five groups (<i>n</i> = 9): Group A received control (untreated feed), Group B received standard bait (0.05% fipronil), and Groups C–E received HSLB with 10%, 20%, or 40% leaf inclusion, respectively. Mortality was assessed at 10 and 24&#xa0;h, along with brain acetylcholinesterase (AChE) activity. HSLB caused a concentration- and time-dependent increase in mortality. AChE activity was significantly elevated in the HSLB-10% group, suggesting a compensatory response to neurophysiological stress and disrupted energy metabolism. To clarify the molecular mechanisms, major constituents of <i>H. suaveolens</i> were docked against <i>P. americana</i> ATP synthase (PaATP synthase) and cytochrome c oxidase (PaCOX), enzymes involved in cellular energy metabolism. Rutin, rosmarinic acid, and hesperidin showed strong binding affinities for PaCOX, while naringenin, caftaric acid, and protocatechuic acid had higher affinities for PaATP synthase. The strong interactions with these mitochondrial targets support the observed toxicity in vivo. The findings suggest that HSLB’s insecticidal effect may involve disruption of ATP production and oxidative phosphorylation, along with neurophysiological changes. Therefore, H. suaveolens may serve as a promising candidate for the development of eco-friendly bioinsecticidal baits for cockroach control.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Experimental and computational evaluation of Hyptis suaveolens leaf-based bait against Periplaneta americana

  • 期刊论文

  • 发表日期：

    2026年8月3日

查看全文
  • Babasoji Percy Omoniwa,
  • Charles Obiora Nwonuma,
  • Titilayo Omolara Johnson,
  • Oluwaseun Felix Oni,
  • Eyitayo Olugbenga Adeyemi,
  • Emenike O. Irokanulo,
  • Abimbola Peter Oluyori,
  • Omolola Soji-Omoniwa,
  • Ifunanyachi Jane-Rose Oche,
  • Olorunleke Oludipe Emmanuel

摘要

Hyptis suaveolens (L.) Poit is known for its insecticidal properties. However, the mechanism of action in bait formulations remains poorly understood. This study evaluated the insecticidal activity of H. suaveolens leaf-based bait (HSLB) against Periplaneta americana using integrated in vivo and in silico approaches. Forty-five cockroaches were randomly assigned into five groups (n = 9): Group A received control (untreated feed), Group B received standard bait (0.05% fipronil), and Groups C–E received HSLB with 10%, 20%, or 40% leaf inclusion, respectively. Mortality was assessed at 10 and 24 h, along with brain acetylcholinesterase (AChE) activity. HSLB caused a concentration- and time-dependent increase in mortality. AChE activity was significantly elevated in the HSLB-10% group, suggesting a compensatory response to neurophysiological stress and disrupted energy metabolism. To clarify the molecular mechanisms, major constituents of H. suaveolens were docked against P. americana ATP synthase (PaATP synthase) and cytochrome c oxidase (PaCOX), enzymes involved in cellular energy metabolism. Rutin, rosmarinic acid, and hesperidin showed strong binding affinities for PaCOX, while naringenin, caftaric acid, and protocatechuic acid had higher affinities for PaATP synthase. The strong interactions with these mitochondrial targets support the observed toxicity in vivo. The findings suggest that HSLB’s insecticidal effect may involve disruption of ATP production and oxidative phosphorylation, along with neurophysiological changes. Therefore, H. suaveolens may serve as a promising candidate for the development of eco-friendly bioinsecticidal baits for cockroach control.