<p>The greenhouse whitefly, <i>Trialeurodes vaporariorum</i>, is a major pest in rose cultivation in Colombia, highlighting the need for effective control methods in export rose crops. Bio-assays were conducted to compare the effects of the insecticides abamectin, dimethoate, imidacloprid, and lambda-cyhalothrin on <i>T. vaporariorum</i> in terms of toxicity, survival, feeding preference, and repellency. Lambda-cyhalothrin showed the highest toxicity (LC₅₀ = 0.02 mg L^–1), followed by imidacloprid (LC₅₀ = 7.22 mg L^–1), abamectin (LC₅₀ = 22.90 mg L^–1), and dimethoate (LC₅₀ = 96.52 mg L^–1). Survival was 99% in the control group and decreased to 45% in whiteflies exposed to the LC₅₀ of dimethoate, 44% for abamectin, 41% for lambda-cyhalothrin, and 38% for imidacloprid. Whiteflies exhibited a lower preference for rose leaves treated with abamectin and lambda-cyhalothrin, while their preference for dimethoate- and imidacloprid-treated leaves remained neutral. Furthermore, dimethoate, imidacloprid, and lambda-cyhalothrin exhibited repellent effects against <i>T. vaporariorum</i>. Overall, abamectin, dimethoate, imidacloprid, and lambda-cyhalothrin caused substantial negative effects on <i>T. vaporariorum</i> and may therefore be considered for inclusion in rotation strategies within integrated pest management programs for this pest in rose crops.</p>