Scholarlink

<p>This study investigates the lethality of three entomopathogenic fungal isolates <i>Beauveria bassiana</i>, <i>Metarhizium anisopliae</i>, and <i>Isaria fumosorosea</i> on different life stages of <i>Scirtothrips dorsalis</i>, a significant agricultural pest. The research was conducted in controlled laboratory conditions at 24 ± 2&#xa0;°C, 55 ± 5% RH, and a 16:8&#xa0;L: D photoperiod. Bioassays were performed by spraying fungal suspensions at concentrations of 1 × 10⁶, 1 × 10⁷, and 1 × 10⁸ conidiospores/mL on the first and second larval stages, pupae, and adult stages of <i>S. dorsalis</i>. Mortality rates were recorded at 1, 3, and 7 days post-inoculation. Each treatment included five replicates of 25 individuals per replicate, ensuring robust data collection. The results revealed that <i>B. bassiana</i> demonstrated the highest lethality, particularly against early larval stages, achieving a maximum mortality rate of 89.4% at the highest concentration. <i>M. anisopliae</i> was highly effective against pupae and adults, with mortality rates reaching 95.2% and 90.7%, respectively. Although <i>I. fumosorosea</i> exhibited moderate lethality, it still showed promising results across all developmental stages, with mortality rates reaching up to 72.1% for first instar larvae. The calculated LC₅₀ and LC₉₀ values confirmed that <i>B. bassiana</i> had the lowest LC₅₀ values for early larval stages, indicating its superior virulence at lower concentrations. These findings highlight the potential of entomopathogenic fungi as effective biological control agents in integrated pest management strategies. Statistical analysis revealed that <i>B. bassiana</i> caused significantly higher mortality in early larval stages compared to <i>M. anisopliae</i> and <i>I. fumosorosea</i>, while <i>M. anisopliae</i> was significantly more lethal to pupae and adults than the other two fungi (<i>P</i> &lt; 0.05). The results underscore the need for further field trials to evaluate environmental factors such as temperature, humidity, and UV exposure, which may influence fungal persistence and lethality in natural settings. Future research should focus on optimizing application methods and assessing potential combinations of fungal isolates for enhanced control outcomes.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

The different stages of Scirtothrips dorsalis (Thysanoptera: Thripidae) exhibit varying levels of susceptibility to entomopathogenic fungal infection in vitro

  • 期刊论文

  • 发表日期：

    2025年11月27日

查看全文
  • Musa Kırışık

摘要

This study investigates the lethality of three entomopathogenic fungal isolates Beauveria bassiana, Metarhizium anisopliae, and Isaria fumosorosea on different life stages of Scirtothrips dorsalis, a significant agricultural pest. The research was conducted in controlled laboratory conditions at 24 ± 2 °C, 55 ± 5% RH, and a 16:8 L: D photoperiod. Bioassays were performed by spraying fungal suspensions at concentrations of 1 × 10⁶, 1 × 10⁷, and 1 × 10⁸ conidiospores/mL on the first and second larval stages, pupae, and adult stages of S. dorsalis. Mortality rates were recorded at 1, 3, and 7 days post-inoculation. Each treatment included five replicates of 25 individuals per replicate, ensuring robust data collection. The results revealed that B. bassiana demonstrated the highest lethality, particularly against early larval stages, achieving a maximum mortality rate of 89.4% at the highest concentration. M. anisopliae was highly effective against pupae and adults, with mortality rates reaching 95.2% and 90.7%, respectively. Although I. fumosorosea exhibited moderate lethality, it still showed promising results across all developmental stages, with mortality rates reaching up to 72.1% for first instar larvae. The calculated LC₅₀ and LC₉₀ values confirmed that B. bassiana had the lowest LC₅₀ values for early larval stages, indicating its superior virulence at lower concentrations. These findings highlight the potential of entomopathogenic fungi as effective biological control agents in integrated pest management strategies. Statistical analysis revealed that B. bassiana caused significantly higher mortality in early larval stages compared to M. anisopliae and I. fumosorosea, while M. anisopliae was significantly more lethal to pupae and adults than the other two fungi (P < 0.05). The results underscore the need for further field trials to evaluate environmental factors such as temperature, humidity, and UV exposure, which may influence fungal persistence and lethality in natural settings. Future research should focus on optimizing application methods and assessing potential combinations of fungal isolates for enhanced control outcomes.