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<p>Bacterial gut symbionts from field and lab-reared larval population of Fall armyworm (FAW), <i>Spodoptera frugiperda</i> on different host plants such as pearl millet, sweet sorghum, groundnut, cotton and artificial diet were isolated and identified based on molecular characterization using 16&#xa0;s rRNA gene sequencing. Fourteen species of bacteria gut symbionts of distinctive characteristics were identified from the FAW larvae collected from field and reared in the lab. The larval population collected from the field harboured more bacterial gut symbionts than lab reared population. The investigation showed Firmicutes and Proteobacteria were the dominant phylum in the FAW larvae collected from the field and lab reared population, respectively. Bacterial gut symbionts from the field-collected larvae were high on pearl millet (12) and sweet sorghum (11), followed by groundnut (9) and cotton (6), whereas lab-reared FAW larvae on pearl millet, sweet sorghum, groundnut, cotton and artificial diet harboured 7, 6, 4, 3 and 7 bacterial gut symbionts, respectively. Bacterial gut symbionts; <i>Pseudomonas stutzeri</i>, <i>Bacillus subtilis</i>, <i>Escherichia coli</i> and <i>Bacillus hyanesii</i> were found only in field collected larval population, whereas <i>Klebsiella variicola</i> and <i>Pseudomonas putida</i> were identified from lab reared population only. The gut symbionts viz., <i>Staphylococcus hominis</i>, <i>Bacillus tequilensis</i>, <i>Pantoea agglomerans</i>, <i>Enterobacter homachei</i>, <i>Bacillus megaterium</i>, <i>Bacillus licheniformis</i><i>, </i><i>Serratia marcescens</i> and <i>Enterococcus casseliflavus</i> were present in both field collected and lab reared larval population of FAW. The identified gut bacteria could be utilised in developing probiotic diets for their potential use in insect nutrition. Dynamic pattern of potential gut symbionts in FAW feeding on different host plants has a significant scope for designing novel pest management strategies like autocidal biological control by manipulating the gut microbiome in target insects.</p>

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Diet-and host-dependent variability in gut bacterial symbionts of the fall armyworm Spodoptera frugiperda (Smith): isolation and molecular identification

  • 期刊论文

  • 发表日期：

    2025年11月27日

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  • H. S. Manohara,
  • G. S. Panduranga,
  • T. Rajasekharam,
  • N. R. Prasanna Kumar

摘要

Bacterial gut symbionts from field and lab-reared larval population of Fall armyworm (FAW), Spodoptera frugiperda on different host plants such as pearl millet, sweet sorghum, groundnut, cotton and artificial diet were isolated and identified based on molecular characterization using 16 s rRNA gene sequencing. Fourteen species of bacteria gut symbionts of distinctive characteristics were identified from the FAW larvae collected from field and reared in the lab. The larval population collected from the field harboured more bacterial gut symbionts than lab reared population. The investigation showed Firmicutes and Proteobacteria were the dominant phylum in the FAW larvae collected from the field and lab reared population, respectively. Bacterial gut symbionts from the field-collected larvae were high on pearl millet (12) and sweet sorghum (11), followed by groundnut (9) and cotton (6), whereas lab-reared FAW larvae on pearl millet, sweet sorghum, groundnut, cotton and artificial diet harboured 7, 6, 4, 3 and 7 bacterial gut symbionts, respectively. Bacterial gut symbionts; Pseudomonas stutzeri, Bacillus subtilis, Escherichia coli and Bacillus hyanesii were found only in field collected larval population, whereas Klebsiella variicola and Pseudomonas putida were identified from lab reared population only. The gut symbionts viz., Staphylococcus hominis, Bacillus tequilensis, Pantoea agglomerans, Enterobacter homachei, Bacillus megaterium, Bacillus licheniformis, Serratia marcescens and Enterococcus casseliflavus were present in both field collected and lab reared larval population of FAW. The identified gut bacteria could be utilised in developing probiotic diets for their potential use in insect nutrition. Dynamic pattern of potential gut symbionts in FAW feeding on different host plants has a significant scope for designing novel pest management strategies like autocidal biological control by manipulating the gut microbiome in target insects.