Scholarlink

<p>The use of karyological studies in insects is highly important. Chromosomal studies and manual karyotyping are traditional yet reliable techniques for determining the identity of a species on an evolutionary scale. However, these techniques are simple, reliable and inexpensive for identifying the existence of a particular species. Species generally possess a fixed number of chromosomes in their cell nuclei, though interspecies differences are common and often significant. These differences could have evolved through multiple speciation events, each involving the fixation of a single chromosomal rearrangement. A brief overview of the current stage of chromosome studies of insect pests belonging to the order <i>Coleoptera</i> is given. In this paper, we outline the use of cytogenetic analysis to study the structure and number of chromosomes in insect cells. Metaphase chromosome spreads were prepared from testicular lobules, and Giemsa staining revealed a diploid chromosome number of 2n = 20 + XYp chromosomes. The karyotype comprises 2 metacentric pairs, 5 submetacentric pairs, and 3 subtelocentric pairs of chromosomes. During the present investigation, supernumerary chromosomes were also observed. The chromosomes ranged in length between 0.28 and 1.08&#xa0;μm, and the total length of the haploid complement was 99.96&#xa0;μm. Based on the absolute length and centromeric positions, the chromosomes were arranged in order of decreasing length in an idiogram. The karyotype formula was 2n= (2&#xa0;m + 5sm + 3st) XYp, including the X submetacentric and Y subtelocentric chromosomes. This study provides valuable insights into the cytogenetic characteristics of <i>Aeolesthes sarta</i> (Solsky, 1871), shedding light on its genetic makeup, evolutionary patterns, and potential mechanisms of adaptation. The findings lay the groundwork for future research aimed at understanding the genetic basis of host preferences and population dynamics and developing targeted control methods for this destructive pest.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Cytogenetic analysis of Aeolesthes Sarta (Solsky, 1871) (Coleoptera: Cerambycidae) the major borer pest on walnut trees (Juglans regia) in Kashmir Valley of J&K, India

  • 期刊论文

  • 发表日期：

    2025年8月22日

查看全文
  • Sajad Ahmad Khan,
  • Sadam Hussain Malik,
  • Inayat Ullah Lone

摘要

The use of karyological studies in insects is highly important. Chromosomal studies and manual karyotyping are traditional yet reliable techniques for determining the identity of a species on an evolutionary scale. However, these techniques are simple, reliable and inexpensive for identifying the existence of a particular species. Species generally possess a fixed number of chromosomes in their cell nuclei, though interspecies differences are common and often significant. These differences could have evolved through multiple speciation events, each involving the fixation of a single chromosomal rearrangement. A brief overview of the current stage of chromosome studies of insect pests belonging to the order Coleoptera is given. In this paper, we outline the use of cytogenetic analysis to study the structure and number of chromosomes in insect cells. Metaphase chromosome spreads were prepared from testicular lobules, and Giemsa staining revealed a diploid chromosome number of 2n = 20 + XYp chromosomes. The karyotype comprises 2 metacentric pairs, 5 submetacentric pairs, and 3 subtelocentric pairs of chromosomes. During the present investigation, supernumerary chromosomes were also observed. The chromosomes ranged in length between 0.28 and 1.08 μm, and the total length of the haploid complement was 99.96 μm. Based on the absolute length and centromeric positions, the chromosomes were arranged in order of decreasing length in an idiogram. The karyotype formula was 2n= (2 m + 5sm + 3st) XYp, including the X submetacentric and Y subtelocentric chromosomes. This study provides valuable insights into the cytogenetic characteristics of Aeolesthes sarta (Solsky, 1871), shedding light on its genetic makeup, evolutionary patterns, and potential mechanisms of adaptation. The findings lay the groundwork for future research aimed at understanding the genetic basis of host preferences and population dynamics and developing targeted control methods for this destructive pest.