Scholarlink

<p>The fall armyworm <i>Spodoptera frugiperda</i> (J.E. Smith) (Lepidoptera: Noctuidae) is a major transnational migratory pest. Flightin is a myosin-binding protein and is critical for maintaining flight muscle integrity and flight function in winged insects. Here, we cloned the <i>flightin</i> gene and analyzed its sequence and function in <i>S. frugiperda</i>. Phylogenetic and motif analyses revealed the conservation and divergence of flightin orthologs in winged insects. The transcript of <i>flightin</i> was knocked down by feeding larvae with bacteria expressing the dsRNA of the target gene, which reduced its transcript levels by 66.73% and 45.94% in 1-d-old female and male adults, respectively. RNAi of <i>flightin</i> significantly reduced the whole body weights of larvae, pupae and adults, and the weight of adult thorax. Flight muscle ultrastructure viewing revealed that RNAi of <i>flightin</i> had significant effects on the structure of flight muscles, with the sarcomere length of RNAi adults being significantly longer than controls. Knockdown of <i>flightin</i> also significantly reduced (&gt; 50%) adult flight capacity in both sexes. These results indicate that flightin plays an important role in the flight function in moths, which may be used to control the flight behavior of this migratory moth pest. This is the first study to determine the function of flightin in lepidopteran.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Knockdown of flightin by bacterial-mediated RNAi affected flight muscle structure and flight capacity in the long-distance migratory moth pest, Spodoptera frugiperda

  • 期刊论文

  • 发表日期：

    2025年5月27日

查看全文
  • He-Wei Sun,
  • Yan Zhang,
  • Wen Fu,
  • Wang-He Zhang,
  • Jun-Zhong Zhang,
  • Jin Xu

摘要

The fall armyworm Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) is a major transnational migratory pest. Flightin is a myosin-binding protein and is critical for maintaining flight muscle integrity and flight function in winged insects. Here, we cloned the flightin gene and analyzed its sequence and function in S. frugiperda. Phylogenetic and motif analyses revealed the conservation and divergence of flightin orthologs in winged insects. The transcript of flightin was knocked down by feeding larvae with bacteria expressing the dsRNA of the target gene, which reduced its transcript levels by 66.73% and 45.94% in 1-d-old female and male adults, respectively. RNAi of flightin significantly reduced the whole body weights of larvae, pupae and adults, and the weight of adult thorax. Flight muscle ultrastructure viewing revealed that RNAi of flightin had significant effects on the structure of flight muscles, with the sarcomere length of RNAi adults being significantly longer than controls. Knockdown of flightin also significantly reduced (> 50%) adult flight capacity in both sexes. These results indicate that flightin plays an important role in the flight function in moths, which may be used to control the flight behavior of this migratory moth pest. This is the first study to determine the function of flightin in lepidopteran.