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<p>Chronic skin ulcers pose a significant clinical burden in patients with diabetes, vascular disease, and other conditions of impaired healing, creating a pressing need for bioactive dressings that actively stimulate regeneration. This study developed and characterized novel bioactive membranes from chitosan (CH) and the triterpenoid madecassoside (MA) to address this need. Hydrogels of 2% CH with 0.10%, 0.25%, and 0.50% MA (CH/MA010, CH/MA025, CH/MA050) were dehydrated into membranes. Comprehensive analysis revealed membranes with high porosity (67–87%), significant fluid absorption capacity (48–88%), and controlled biodegradation. The membranes exhibited a potent, non-diffusible, contact-based antimicrobial effect, completely inhibiting the growth of <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> underneath them. In vitro biological evaluation demonstrated that all membranes supported fibroblast (NIH/3T3) viability. Crucially, the CH/MA membranes significantly enhanced fibroblast migration in a scratch-wound assay. The CH/MA025 formulation emerged as the lead candidate, demonstrating optimal cytocompatibility and the most consistent and significant promotion of wound closure. These results indicate that the incorporation of 0.25% madecassoside creates a synergistic effect, transforming chitosan into a multifunctional membrane that not only manages the wound environment but also actively stimulates regeneration. The CH/MA025 composite membrane is therefore a highly promising biomaterial for the treatment of chronic skin ulcers.</p>

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Characterization of chitosan matrix associated with madecassoside for skin healing

  • 期刊论文

  • 发表日期：

    2025年12月9日

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  • Paola Silva Cardoso,
  • Florença Sayuri Mikawa dos Santos,
  • Graziela Aparecida Silva Gonçalves,
  • Maria Renata Sales Nogueira

摘要

Chronic skin ulcers pose a significant clinical burden in patients with diabetes, vascular disease, and other conditions of impaired healing, creating a pressing need for bioactive dressings that actively stimulate regeneration. This study developed and characterized novel bioactive membranes from chitosan (CH) and the triterpenoid madecassoside (MA) to address this need. Hydrogels of 2% CH with 0.10%, 0.25%, and 0.50% MA (CH/MA010, CH/MA025, CH/MA050) were dehydrated into membranes. Comprehensive analysis revealed membranes with high porosity (67–87%), significant fluid absorption capacity (48–88%), and controlled biodegradation. The membranes exhibited a potent, non-diffusible, contact-based antimicrobial effect, completely inhibiting the growth of Escherichia coli and Staphylococcus aureus underneath them. In vitro biological evaluation demonstrated that all membranes supported fibroblast (NIH/3T3) viability. Crucially, the CH/MA membranes significantly enhanced fibroblast migration in a scratch-wound assay. The CH/MA025 formulation emerged as the lead candidate, demonstrating optimal cytocompatibility and the most consistent and significant promotion of wound closure. These results indicate that the incorporation of 0.25% madecassoside creates a synergistic effect, transforming chitosan into a multifunctional membrane that not only manages the wound environment but also actively stimulates regeneration. The CH/MA025 composite membrane is therefore a highly promising biomaterial for the treatment of chronic skin ulcers.