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<p>The present study was conducted to evaluate the genetic diversity using agro-morphological traits and molecular markers. Agro-morphological traits such as collar diameter, leaflet length and leaflet width revealed significant genetic variation in the population. PCR-based molecular markers, viz., Random Amplified Polymorphism DNA (RAPD) and inter simple sequence repeat (ISSR), were employed to assess population structure and DNA fingerprinting in karanja accessions. The RAPD marker revealed 66.87% polymorphism, with the OPE-9 marker showing 100% polymorphism and a high polymorphism information content (PIC) of 0.65. The ISSR marker exhibited 38.35% polymorphism, with UBC-850 targeting (GT) repeats demonstrating the highest PIC of 0.23, while UBC-827 was highly polymorphic at 72.72%. The RAPD marker showed a higher primer index, PIC, and marker index compared to the ISSR marker. Population structure analysis revealed two main groups with three subgroups. DNA fingerprinting identified a unique haplotype pattern that distinguished every individual in the karanja population. The diverse karanja accessions identified could be effectively utilized as divergent parents in future breeding programs to exploit the heterosis phenomenon.</p>

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Exploring genetic diversity, population structure and DNA fingerprinting of ex situ established Karanja (Derris indica L.) germplasms

  • 期刊论文

  • 发表日期：

    2026年6月15日

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  • Anil A Hake,
  • Sanjay Jha,
  • Suman Kumar Jha,
  • Mahesh K Mahatma,
  • Kiran P Suthar

摘要

The present study was conducted to evaluate the genetic diversity using agro-morphological traits and molecular markers. Agro-morphological traits such as collar diameter, leaflet length and leaflet width revealed significant genetic variation in the population. PCR-based molecular markers, viz., Random Amplified Polymorphism DNA (RAPD) and inter simple sequence repeat (ISSR), were employed to assess population structure and DNA fingerprinting in karanja accessions. The RAPD marker revealed 66.87% polymorphism, with the OPE-9 marker showing 100% polymorphism and a high polymorphism information content (PIC) of 0.65. The ISSR marker exhibited 38.35% polymorphism, with UBC-850 targeting (GT) repeats demonstrating the highest PIC of 0.23, while UBC-827 was highly polymorphic at 72.72%. The RAPD marker showed a higher primer index, PIC, and marker index compared to the ISSR marker. Population structure analysis revealed two main groups with three subgroups. DNA fingerprinting identified a unique haplotype pattern that distinguished every individual in the karanja population. The diverse karanja accessions identified could be effectively utilized as divergent parents in future breeding programs to exploit the heterosis phenomenon.