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<p><i>Crassocephalum crepidioides</i> is an ethnomedicinal plant traditionally used for the treatment of various ailments and is widely consumed as a vegetable in certain parts of Northeast India and globally. The present study aims to determine the antioxidant and antimicrobial potential of extracts obtained from n-hexane, butanol, ethyl acetate, and methanol, and to identify the bioactive substances present in <i>C. crepidioides.</i> Preliminary phytochemical screening showed the presence of alkaloids, flavonoids, tannins, phytosterols, saponins, carbohydrates, and reducing sugars. The butanol extract exhibited the highest TPC (55.65 ± 0.008&#xa0;mg/g) and TFC (46.98 ± 0.003&#xa0;mg/g), as well as the highest antioxidant potential, with values of 27.04 ± 0.993&#xa0;mg/mL and 24.9 ± 2.379&#xa0;mg/mL against DPPH and ABTS free radicals, respectively. Furthermore, the butanol extract also showed the best antimicrobial activity. Since butanol extract showed high bioactive potential, it was chosen for further phytochemical analysis. Approximately six different classes of compounds were detected by GC-MS analysis, and ten different classes of compounds by LC-MS analysis. Our findings suggest that <i>C. crepidioides</i> is a promising therapeutic agent for a range of biomedical applications due to its strong antibacterial and antioxidant properties, as well as its numerous bioactive components. Thus, the present study provides a useful framework for further research into the functional roles and bioactivities of these compound classes.</p>

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Phytochemical profiling and bioactive assessment of Crassocephalum crepidiodes using GC-MS and LC-MS analysis

  • 期刊论文

  • 发表日期：

    2026年4月22日

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  • B. C. Lalremruata,
  • Nick Lalrinmawia,
  • Garima Singh

摘要

Crassocephalum crepidioides is an ethnomedicinal plant traditionally used for the treatment of various ailments and is widely consumed as a vegetable in certain parts of Northeast India and globally. The present study aims to determine the antioxidant and antimicrobial potential of extracts obtained from n-hexane, butanol, ethyl acetate, and methanol, and to identify the bioactive substances present in C. crepidioides. Preliminary phytochemical screening showed the presence of alkaloids, flavonoids, tannins, phytosterols, saponins, carbohydrates, and reducing sugars. The butanol extract exhibited the highest TPC (55.65 ± 0.008 mg/g) and TFC (46.98 ± 0.003 mg/g), as well as the highest antioxidant potential, with values of 27.04 ± 0.993 mg/mL and 24.9 ± 2.379 mg/mL against DPPH and ABTS free radicals, respectively. Furthermore, the butanol extract also showed the best antimicrobial activity. Since butanol extract showed high bioactive potential, it was chosen for further phytochemical analysis. Approximately six different classes of compounds were detected by GC-MS analysis, and ten different classes of compounds by LC-MS analysis. Our findings suggest that C. crepidioides is a promising therapeutic agent for a range of biomedical applications due to its strong antibacterial and antioxidant properties, as well as its numerous bioactive components. Thus, the present study provides a useful framework for further research into the functional roles and bioactivities of these compound classes.