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<p>Consistent measurement of DNA concentration and purity is vital for all molecular studies. In the current extraction protocol, 0.5&#xa0;g of juvenile leaf tissues were used to check the efficiency of DNA extraction. The quality and quantity of genomic DNA were tested on 0.8% agarose gel and a UV-visible spectrophotometer. The suitability of extracted genomic DNA for its use in molecular marker assays, particularly Simple Sequence Repeats. The DNA yield ranged from 306 to 1693 ng/µL, with an absorbance ratio between 1.75−1.92, respectively. In this protocol, elevated concentrations of CTAB, PVP, and β-mercaptoethanol were used to effectively remove proteins, tannins, and polyphenols. The effective PCR amplification of the leaf tissues confirmed that the genomic DNA was of good quality at the initial concentration of 40ng/ µL. Thus, the modified CTAB method (M2) can therefore be employed to extract high-quality genomic DNA from juvenile leaf tissues of <i>T. ciliata</i>, which predominantly contain proteins and tannins.</p>

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An efficient genomic DNA isolation protocol from juvenile leaves of Toona ciliata M. Roem

  • 期刊论文

  • 发表日期：

    2026年2月22日

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  • Neha,
  • Rajendra K. Meena,
  • Maneesh S. Bhandari,
  • Rimpee Garg,
  • Abhishek Yadav,
  • Vipul Sharma,
  • Rama Kant

摘要

Consistent measurement of DNA concentration and purity is vital for all molecular studies. In the current extraction protocol, 0.5 g of juvenile leaf tissues were used to check the efficiency of DNA extraction. The quality and quantity of genomic DNA were tested on 0.8% agarose gel and a UV-visible spectrophotometer. The suitability of extracted genomic DNA for its use in molecular marker assays, particularly Simple Sequence Repeats. The DNA yield ranged from 306 to 1693 ng/µL, with an absorbance ratio between 1.75−1.92, respectively. In this protocol, elevated concentrations of CTAB, PVP, and β-mercaptoethanol were used to effectively remove proteins, tannins, and polyphenols. The effective PCR amplification of the leaf tissues confirmed that the genomic DNA was of good quality at the initial concentration of 40ng/ µL. Thus, the modified CTAB method (M2) can therefore be employed to extract high-quality genomic DNA from juvenile leaf tissues of T. ciliata, which predominantly contain proteins and tannins.