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<p><i>Solanum villosum</i> Mill., an underutilized ethnomedicinal plant of Solanaceae, distributed across India and worldwide, is promising for commercial exploitation as an alternative food and medicinal resource for sustainable future. This study was conducted to achieve continuous production of the bioactive compound solasodine from the in vitro mass-propagated plants of the selected elite chemotype of <i>S. villosum</i>. Plant collections were conducted from 30 distinct agroecological regions of West Bengal to identify the best chemotype based on solasodine content, analyzed through high-performance liquid chromatography (HPLC). Among the different accessions, chemotype SV-HW-05 exhibited maximum solasodine concentration (0.64 ± 0.06&#xa0;mg/g DW) and was thus selected as the elite mother plant for in vitro regeneration. Optimal shoot multiplication was achieved in MS medium supplemented with 1.0&#xa0;mg/l <i>m</i>T (<i>meta</i>-Topolin) and 0.5&#xa0;mg/l NAA (α-naphthaleneacetic acid), which produced the maximum number (54.65 ± 0.15) of shoots with mean shoot length of 3.54 ± 0.29&#xa0;cm. Best root induction from regenerated shoots was achieved in ½ MS medium supplemented with 0.5&#xa0;mg/l IBA (indole-3-butyric acid), which produced 48.53 ± 0.15 number of roots per microshoot and mean root length of 3.97 ± 0.39&#xa0;cm. The regenerated plantlets were acclimatized to ex vitro conditions, and their bioactive compound production was compared with the mother plant for phytochemical stability. Genetic fidelity was confirmed by start codon targeted (SCoT) polymorphism analysis. This study offers a straightforward, validated, and reproducible approach for the commercially viable mass-scale cultivation of elite <i>S. villosum</i> chemotypes, for enhancing solasodine production from this ethnomedicinal plant.</p>

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Elite chemotype selection and meta-Topolin (mT) mediated mass propagation of underutilized medicinal plant Solanum villosum Mill. for sustainable production of the bioactive compound Solasodine

  • 期刊论文

  • 发表日期：

    2026年1月19日

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  • Tarun Halder,
  • Diptesh Biswas,
  • Md. Mabud Alam,
  • Sk. Moquammel Haque,
  • Biswajit Ghosh

摘要

Solanum villosum Mill., an underutilized ethnomedicinal plant of Solanaceae, distributed across India and worldwide, is promising for commercial exploitation as an alternative food and medicinal resource for sustainable future. This study was conducted to achieve continuous production of the bioactive compound solasodine from the in vitro mass-propagated plants of the selected elite chemotype of S. villosum. Plant collections were conducted from 30 distinct agroecological regions of West Bengal to identify the best chemotype based on solasodine content, analyzed through high-performance liquid chromatography (HPLC). Among the different accessions, chemotype SV-HW-05 exhibited maximum solasodine concentration (0.64 ± 0.06 mg/g DW) and was thus selected as the elite mother plant for in vitro regeneration. Optimal shoot multiplication was achieved in MS medium supplemented with 1.0 mg/l mT (meta-Topolin) and 0.5 mg/l NAA (α-naphthaleneacetic acid), which produced the maximum number (54.65 ± 0.15) of shoots with mean shoot length of 3.54 ± 0.29 cm. Best root induction from regenerated shoots was achieved in ½ MS medium supplemented with 0.5 mg/l IBA (indole-3-butyric acid), which produced 48.53 ± 0.15 number of roots per microshoot and mean root length of 3.97 ± 0.39 cm. The regenerated plantlets were acclimatized to ex vitro conditions, and their bioactive compound production was compared with the mother plant for phytochemical stability. Genetic fidelity was confirmed by start codon targeted (SCoT) polymorphism analysis. This study offers a straightforward, validated, and reproducible approach for the commercially viable mass-scale cultivation of elite S. villosum chemotypes, for enhancing solasodine production from this ethnomedicinal plant.