Bioactivities and metabolites profiling of different solvent extracts of novel Poly-Herbal Formulation used by tea tribes of Upper Assam
摘要
The aim of this study is to scientifically validate a novel Poly-Herbal Formulation (PHF) used by tribal communities of Upper Assam in treating respiratory diseases like pneumonia. The PHF is constituted by distinctive parts of four plants, barks of Azadirachta indica, seeds of Caesalpinia bonduc, roots of Solanum spirale and rhizomes of Cyperus rotundus grinded together in the ratio 1: 0.3: 1: 1. The different solvent extracts exhibited IC50 values in the range of 49.58–84.78 µg/ml (DPPH assay), 81.98–116.88 µg/ml (Nitric Oxide Scavenging Assay) and 4.023–8.243 mg/ml (Total Anti-oxidant assay) while the Zone of Inhibition (ZOI) for E. coli, P. aeruginosa and C. albicans measured within the range of 15–31 mm. Minimum Inhibitory Concentration (MIC) of the methanolic extract measured 0.002 mg/ml for E. coli, 0.002 mg/ml for P. aeruginosa and 0.012 mg/ml for C. albicans. Phenolic and flavonoid content analysis of methanolic extract revealed values equaling 63 ± 0.002 mg Gallic Acid Equivalent per 100 mg plant extract for phenolic content and 9.7 mg quercetin equivalent per 100 mg plant extract for flavonoid content. The FTIR of methanolic extract revealed the presence of functional characteristic peaks of ester, amine, acid, alcohol, alkane, alkene and aromatic compounds. Furthermore the GC–MS analysis revealed the presence of 10 phyto-constituents while some like 4H-Pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl- possess therapeutic properties like anti-oxidant, anti-microbial and anti-proliferative. Thus the PHF is a natural source of anti-oxidants and anti-microbial bioactive compounds.