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<p>This study provides a comprehensive investigation to identify the phylogenetic classification of <i>Cistanche violacea</i> using internal transcribed spacer sequencing. Bioactive phytocompounds were screened using gas chromatography-mass spectrometry (GC–MS) and FTIR spectroscopy. Additionally, the antioxidant (ABTS, DPPH assay, and the total antioxidant capacity (TAC)) and antibacterial (MIC method) properties of the crude extract and fractions prepared from four solvents with increasing polarity were explored. Results revealed that chloroform, ethyl acetate, and dichloromethane exhibited strong antioxidant activities toward DPPH (IC50 = 15.46 ± 0.15&#xa0;µg/ml), ABTS (IC50 = 2.94 ± 0.34&#xa0;µg/ml), and TAC (IC50 = 1.65 ± 0.004&#xa0;µg/ml), respectively. GC–MS and FTIR analysis identified several functional groups and promising bioactive molecules in the DCM, ethyl acetate, and chloroform fractions. Notably, cyclodecane (in chloroform) and cyclopropanes (in ethyl acetate). Concerning antibacterial activity, most tested extracts were particularly effective against <i>Listeria monocytogenes</i> ATCC 19115 and had suppressed methicillin-resistant <i>Staphylococcus aureus</i> ATCC 43300 more effectively than gentamicin. These findings provide concrete evidence for the biological potential of <i>C. violacea</i> constituents.</p>

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GC–MS and FTIR characterization, unveiling phenolic content, antioxidant and antibacterial properties of the parasitic plant Cistanche violacea

  • 期刊论文

  • 发表日期：

    2025年11月26日

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  • Siham Kara-Zaitri,
  • Farid Berroukeche,
  • Nadia Aissaoui,
  • Sarra Abbad,
  • Fodil Aoulmi,
  • Abdelilah Amrouche

摘要

This study provides a comprehensive investigation to identify the phylogenetic classification of Cistanche violacea using internal transcribed spacer sequencing. Bioactive phytocompounds were screened using gas chromatography-mass spectrometry (GC–MS) and FTIR spectroscopy. Additionally, the antioxidant (ABTS, DPPH assay, and the total antioxidant capacity (TAC)) and antibacterial (MIC method) properties of the crude extract and fractions prepared from four solvents with increasing polarity were explored. Results revealed that chloroform, ethyl acetate, and dichloromethane exhibited strong antioxidant activities toward DPPH (IC50 = 15.46 ± 0.15 µg/ml), ABTS (IC50 = 2.94 ± 0.34 µg/ml), and TAC (IC50 = 1.65 ± 0.004 µg/ml), respectively. GC–MS and FTIR analysis identified several functional groups and promising bioactive molecules in the DCM, ethyl acetate, and chloroform fractions. Notably, cyclodecane (in chloroform) and cyclopropanes (in ethyl acetate). Concerning antibacterial activity, most tested extracts were particularly effective against Listeria monocytogenes ATCC 19115 and had suppressed methicillin-resistant Staphylococcus aureus ATCC 43300 more effectively than gentamicin. These findings provide concrete evidence for the biological potential of C. violacea constituents.