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<p>Plant glutathione S-transferases (GSTs) are multifunctional conserved protein superfamily that is involved in various biological processes such as growth and development, cellular detoxification, stress biology, and various signaling processes. In the current study, a comprehensive genome-wide identification and characterization of the GST gene family were performed in the agriculturally important legume crop <i>Cajanus cajan.</i> A total of 68 GST genes were identified that belong to eight GST classes based on their conserved domains and motifs. Among 68 CcGST genes, 37 CcGST genes were found on seven <i>Cajanus</i> chromosomes and the remaining CcGST genes were found on the scaffold. Both segmental and tandem duplications were key drivers of CcGST gene family expansion. The conserved pattern of exon and intron structure among the different GST classes was observed. The physicochemical properties of major CcGST proteins reveal that they are acidic in nature. The expression profiling study revealed the high expression of CcGSTU38, CcGSTU40, CcGSTU44, CcGSTL3, CcGSTL4, CcEF1G1, CcEF1G2, CcDHAR2 and CcGSTF6 in most of the developmental stages in different anatomical tissues. The molecular docking and MD simulation study of highly expressed CcGSTU38 with the herbicide safeners revealed its highest binding affinity and structural stability with Fenclorim (-5.44&#xa0;kcal/mol). This gene could be a potential candidate for future molecular characterization under herbicide stress. The results of the current study endow us with the further functional analysis of <i>Cajanus</i> GSTs in the future.</p>

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Identification and characterization of GSTase gene family in different developmental stages of C. cajan using various software and computational tools of plant genome biology

  • 期刊论文

  • 发表日期：

    2025年8月19日

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  • Nootan Singh,
  • Sumanta Kumar Sahu,
  • Rajesh Kondabala,
  • Swati Vaish

摘要

Plant glutathione S-transferases (GSTs) are multifunctional conserved protein superfamily that is involved in various biological processes such as growth and development, cellular detoxification, stress biology, and various signaling processes. In the current study, a comprehensive genome-wide identification and characterization of the GST gene family were performed in the agriculturally important legume crop Cajanus cajan. A total of 68 GST genes were identified that belong to eight GST classes based on their conserved domains and motifs. Among 68 CcGST genes, 37 CcGST genes were found on seven Cajanus chromosomes and the remaining CcGST genes were found on the scaffold. Both segmental and tandem duplications were key drivers of CcGST gene family expansion. The conserved pattern of exon and intron structure among the different GST classes was observed. The physicochemical properties of major CcGST proteins reveal that they are acidic in nature. The expression profiling study revealed the high expression of CcGSTU38, CcGSTU40, CcGSTU44, CcGSTL3, CcGSTL4, CcEF1G1, CcEF1G2, CcDHAR2 and CcGSTF6 in most of the developmental stages in different anatomical tissues. The molecular docking and MD simulation study of highly expressed CcGSTU38 with the herbicide safeners revealed its highest binding affinity and structural stability with Fenclorim (-5.44 kcal/mol). This gene could be a potential candidate for future molecular characterization under herbicide stress. The results of the current study endow us with the further functional analysis of Cajanus GSTs in the future.