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<p>Nimbadi Churna (NC) is a classical polyherbal Ayurvedic formulation mentioned in the Ayurvedic Pharmacopoeia of India (API) and Ayurvedic Formulary of India (AFI), recommended for the treatment of <i>kustha</i> (skin diseases), <i>udara</i> (abdomen diseases), <i>vatarakta</i> (gout), <i>amavata</i> (rheumatism). The present study aimed to establish the quality standards for Nimbadi Churna with comprehensive quality control analysis by estimating markers qualitative and quantitatively. Phytochemical, aflatoxin, microbial load and elemental analysis of NC formulation were done as per standard procedures. Qualitative marker analysis was done using HPTLC and GC-MS. Quantitative marker analysis was done by HPLC. In HPTLC analysis, with a combination of toluene: ethyl acetate: formic acid (10:6:0.2 v/v/v) solvent system marker compounds piperlongumine, gallic acid, piperine, curcumin and thymol were found in NC. GC-MS analysis revealed β-asarone (32.96%), α-asarone (28.63%) in volatile oil of NC. In HPLC analysis, piperine was quantified using acetonitrile: water (80:20 v/v) gradient elution mobile phase and the amount of piperine found in NC is 0.0256%. Similarly, the amount of ellagic acid, gallic acid, piperlongumine and embelin in NC were quantified and the values are 0.0137%, 0.0117%, 0.0047% and 0.0003% respectively. The developed standard parameters for NC can be employed as a quality control tool for ensuring proper quality checks by Ayurvedic stakeholders. Nimbadi Churna, quality control, high-performance thinlayer chromatography, high-performance liquid chromatography.</p>

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A comprehensive quality control study, marker analysis by HPTLC, HPLC and GC-MS in the course of development of quality standards for Nimbadi Churna: an ayurvedic poly herbal formulation

  • 期刊论文

  • 发表日期：

    2025年8月19日

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  • Venkata Narasimhaji Ch,
  • Prathapa Reddy Maramreddy,
  • Rajesh Bolleddu,
  • Yashika Gandhi,
  • Dattatray Dighe,
  • Subhose Varanasi,
  • Manosi Das,
  • Meda M. Rao,
  • Das Debajyoti,
  • Ravindra Singh,
  • Babu Gajji,
  • Narayanam Srikanth,
  • Rabinarayan Acharya

摘要

Nimbadi Churna (NC) is a classical polyherbal Ayurvedic formulation mentioned in the Ayurvedic Pharmacopoeia of India (API) and Ayurvedic Formulary of India (AFI), recommended for the treatment of kustha (skin diseases), udara (abdomen diseases), vatarakta (gout), amavata (rheumatism). The present study aimed to establish the quality standards for Nimbadi Churna with comprehensive quality control analysis by estimating markers qualitative and quantitatively. Phytochemical, aflatoxin, microbial load and elemental analysis of NC formulation were done as per standard procedures. Qualitative marker analysis was done using HPTLC and GC-MS. Quantitative marker analysis was done by HPLC. In HPTLC analysis, with a combination of toluene: ethyl acetate: formic acid (10:6:0.2 v/v/v) solvent system marker compounds piperlongumine, gallic acid, piperine, curcumin and thymol were found in NC. GC-MS analysis revealed β-asarone (32.96%), α-asarone (28.63%) in volatile oil of NC. In HPLC analysis, piperine was quantified using acetonitrile: water (80:20 v/v) gradient elution mobile phase and the amount of piperine found in NC is 0.0256%. Similarly, the amount of ellagic acid, gallic acid, piperlongumine and embelin in NC were quantified and the values are 0.0137%, 0.0117%, 0.0047% and 0.0003% respectively. The developed standard parameters for NC can be employed as a quality control tool for ensuring proper quality checks by Ayurvedic stakeholders. Nimbadi Churna, quality control, high-performance thinlayer chromatography, high-performance liquid chromatography.