Scholarlink

<p><i>Alstonia scholaris</i> (L.) R. Br. is used in the Mizo traditional medicine exclusively as a remedy for parasitic infections such as malaria and intestinal helminthiasis. The study attempted to test its biological activities against an intestinal helminth, <i>Raillietina tetragona</i>, and two isolates of <i>Plasmodium falciparum</i>, multidrug-resistant K1 and drug-sensitive 3D7. An aqueous extract of the bark was used to treat cestode and malarial parasites. Chemical analysis was performed with gas chromatography-mass spectrometry, and the principal compound were screened in silico using key proteins of helminths and <i>Plasmodium.</i> The plant extract showed concentration-dependent activity against the cestode parasite. Histology revealed structural breakdown in the parasite including tegumental collapse, disintegration of musculature and dissociation of the eggs. Under scanning electron microscopy, tegumental shrinkage and erosion, bleb formation, removal of spines and microtriches were discernible. The plant extract exhibited antimalarial activity against <i>P. falciparum</i> but with moderate efficacy at the inhibitory concentration (IC<sub>50</sub>) above 50&#xa0;µg/mL. It was equally effective against the drug-resistant isolate. Cytotoxicity test against primate cell line (VERO C1008) showed a half-maximal cytotoxic concentration (CC<sub>50</sub>) at &gt; 100&#xa0;µg/mL, indicating that it is highly non-toxic and safe for therapeutic use. Gelsemine and 7,9-di-tert-butyl-1-oxaspiro(4,5)deca-6,9-diene-2,8-dione were identified as the principal phytocompounds. The compounds revealed high ligand-binding potentials against helminth proteins such as β-tubulin and glutamate-gated chloride channel (GluCl), and malarial proteins like <i>Plasmodium falciparum</i> erythrocyte membrane protein 1 (VAR2CSA) and S-adenosyl-L-homocysteine hydrolase (SAHH). Gelsemine was found to have exceptionally high binding efficiency on GluCl and VAR2CSA in terms of binding energy and amino acid interactions. The findings substantiate the traditional use of <i>A. scholaris</i> and highlight its potential as a source of novel antiparasitic agents.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Pharmacological investigation on the antiparasitic properties of Alstonia scholaris focussing on the antiplasmodial and anticestodal uses in the Mizo traditional medicine of India

  • 期刊论文

  • 发表日期：

    2025年7月19日

查看全文
  • Lalngaihmanawmi,
  • Pawi Bawitlung Lalthanpuii,
  • Bawitlung Lalruatfela,
  • Lalnundanga,
  • Kholhring Lalchhandama

摘要

Alstonia scholaris (L.) R. Br. is used in the Mizo traditional medicine exclusively as a remedy for parasitic infections such as malaria and intestinal helminthiasis. The study attempted to test its biological activities against an intestinal helminth, Raillietina tetragona, and two isolates of Plasmodium falciparum, multidrug-resistant K1 and drug-sensitive 3D7. An aqueous extract of the bark was used to treat cestode and malarial parasites. Chemical analysis was performed with gas chromatography-mass spectrometry, and the principal compound were screened in silico using key proteins of helminths and Plasmodium. The plant extract showed concentration-dependent activity against the cestode parasite. Histology revealed structural breakdown in the parasite including tegumental collapse, disintegration of musculature and dissociation of the eggs. Under scanning electron microscopy, tegumental shrinkage and erosion, bleb formation, removal of spines and microtriches were discernible. The plant extract exhibited antimalarial activity against P. falciparum but with moderate efficacy at the inhibitory concentration (IC50) above 50 µg/mL. It was equally effective against the drug-resistant isolate. Cytotoxicity test against primate cell line (VERO C1008) showed a half-maximal cytotoxic concentration (CC50) at > 100 µg/mL, indicating that it is highly non-toxic and safe for therapeutic use. Gelsemine and 7,9-di-tert-butyl-1-oxaspiro(4,5)deca-6,9-diene-2,8-dione were identified as the principal phytocompounds. The compounds revealed high ligand-binding potentials against helminth proteins such as β-tubulin and glutamate-gated chloride channel (GluCl), and malarial proteins like Plasmodium falciparum erythrocyte membrane protein 1 (VAR2CSA) and S-adenosyl-L-homocysteine hydrolase (SAHH). Gelsemine was found to have exceptionally high binding efficiency on GluCl and VAR2CSA in terms of binding energy and amino acid interactions. The findings substantiate the traditional use of A. scholaris and highlight its potential as a source of novel antiparasitic agents.