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<p>The present work focuses on the in vitro regeneration of <i>Pelatantheria insectifera</i> (Rchb.f.) Ridl, an endangered medicinal orchid, using meta-topolin (mT) and genetic fidelity to validate the multiplication strategy. This plant possesses a potential source of bioactive compounds that can be used for treating various illnesses associated with inflammatory conditions. Mass propagation of <i>P. insectifera</i> is important because of these properties, and it has also been listed as endangered under CITES due to habitat destruction and poor regeneration in the wild. The half-strength Murashige and Skoog’s (MS) medium supplemented with mT exhibited superior shoot growth compared to BAP. The optimal shoot proliferation (4.93 shoots per explant) occurred on the medium containing 1&#xa0;mg/L mT, with the longest shoot (0.91&#xa0;cm) found on the medium with 1.5&#xa0;mg/L mT. The optimal number of leaves (3.30) was recorded on medium supplemented with 2&#xa0;mg/L mT. The synergistic effect of mT or BAP combined with auxin (NAA) on shoot proliferation was assessed, with mT (1.5&#xa0;mg/L) + NAA (0.5&#xa0;mg/L) inducing maximum shoots per explant (17.66). Optimal in vitro rooting was achieved with 1.5&#xa0;mg/L IBA, yielding the highest root count (6.10) and the greatest root length (7.02&#xa0;cm). The clonal fidelity analysis with seven SCoT and five ISSR markers demonstrated 100% monomorphic banding patterns between the micropropagated plantlets and the mother plant. This simplified approach has the potential to propagate true-to-type planting material on a wide scale to reduce pressure on natural resources and restore natural populations of endangered commercially and medicinally valuable orchids.</p>

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Meta-topolin enhanced in vitro regeneration and genetic fidelity assessment of an endangered orchid, Pelatantheria insectifera using scot and ISSR

  • 期刊论文

  • 发表日期：

    2025年7月9日

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  • Kangabam Soneja Devi,
  • Heisnam Haripriyari Devi,
  • Nandeibam Samarjit Singh,
  • Huidrom Sunitibala Devi

摘要

The present work focuses on the in vitro regeneration of Pelatantheria insectifera (Rchb.f.) Ridl, an endangered medicinal orchid, using meta-topolin (mT) and genetic fidelity to validate the multiplication strategy. This plant possesses a potential source of bioactive compounds that can be used for treating various illnesses associated with inflammatory conditions. Mass propagation of P. insectifera is important because of these properties, and it has also been listed as endangered under CITES due to habitat destruction and poor regeneration in the wild. The half-strength Murashige and Skoog’s (MS) medium supplemented with mT exhibited superior shoot growth compared to BAP. The optimal shoot proliferation (4.93 shoots per explant) occurred on the medium containing 1 mg/L mT, with the longest shoot (0.91 cm) found on the medium with 1.5 mg/L mT. The optimal number of leaves (3.30) was recorded on medium supplemented with 2 mg/L mT. The synergistic effect of mT or BAP combined with auxin (NAA) on shoot proliferation was assessed, with mT (1.5 mg/L) + NAA (0.5 mg/L) inducing maximum shoots per explant (17.66). Optimal in vitro rooting was achieved with 1.5 mg/L IBA, yielding the highest root count (6.10) and the greatest root length (7.02 cm). The clonal fidelity analysis with seven SCoT and five ISSR markers demonstrated 100% monomorphic banding patterns between the micropropagated plantlets and the mother plant. This simplified approach has the potential to propagate true-to-type planting material on a wide scale to reduce pressure on natural resources and restore natural populations of endangered commercially and medicinally valuable orchids.