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<p>The present research focused on the identification and characterization of butterfly pea (<i>Clitoria ternatea</i> L.), employing a combination of morphological, micromorphological, biochemical, and molecular markers-based studies. Morphological characterization of six germplasms (Blue Single, White Single, Blue Double, White Double, Purple Single, and Purple Double) of butterfly pea revealed that two germplasms, namely, Blue Single and White Single, were similar in almost all morphological features but significantly different from the other four germplasms. Biochemical analyses on total phenol content and five antioxidant properties (DPPH, ABTS, FRAP assays, SOD, and POD activities) exhibited significant levels of variation across the germplasms. HPLC-based cyanidin quantification indicated substantial diversity among the germplasms, with the highest cyanidin content (773.43&#xa0;mg/100&#xa0;g fresh weight) was found in Purple Double. Flow cytometry-based comparison of ploidy levels revealed a double peak in the Purple Double, unlike a single peak in the other five germplasms, suggesting the possibility of mixoploidy in it. Additionally, molecular marker-based diversity was assessed using inter simple sequence repeats and start codon targeted polymorphism markers, resulting in 94 polymorphic bands, indicating 14.78% polymorphism. Purple Double was found to be completely distinct from the other germplasms and was placed separately in a different root cluster, making it a potential candidate for hybridization. This comprehensive analysis highlights the potential of butterfly pea for breeding programs, especially with the identified genetic diversity and distinct characteristics in the Purple Double germplasm.</p>

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Unveiling genetic diversity and characterization of butterfly pea (Clitoria ternatea L.) germplasms using morphometric, biochemical, flow cytometry, and molecular markers

  • 期刊论文

  • 发表日期：

    2025年6月23日

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  • Anwesa Bhusan,
  • Jayoti Majumder Sarkar,
  • Tsama Subrahmanyeswari,
  • Sandipan Bandyopadhyay,
  • Tapas Kumar Chowdhuri,
  • Saikat Gantait

摘要

The present research focused on the identification and characterization of butterfly pea (Clitoria ternatea L.), employing a combination of morphological, micromorphological, biochemical, and molecular markers-based studies. Morphological characterization of six germplasms (Blue Single, White Single, Blue Double, White Double, Purple Single, and Purple Double) of butterfly pea revealed that two germplasms, namely, Blue Single and White Single, were similar in almost all morphological features but significantly different from the other four germplasms. Biochemical analyses on total phenol content and five antioxidant properties (DPPH, ABTS, FRAP assays, SOD, and POD activities) exhibited significant levels of variation across the germplasms. HPLC-based cyanidin quantification indicated substantial diversity among the germplasms, with the highest cyanidin content (773.43 mg/100 g fresh weight) was found in Purple Double. Flow cytometry-based comparison of ploidy levels revealed a double peak in the Purple Double, unlike a single peak in the other five germplasms, suggesting the possibility of mixoploidy in it. Additionally, molecular marker-based diversity was assessed using inter simple sequence repeats and start codon targeted polymorphism markers, resulting in 94 polymorphic bands, indicating 14.78% polymorphism. Purple Double was found to be completely distinct from the other germplasms and was placed separately in a different root cluster, making it a potential candidate for hybridization. This comprehensive analysis highlights the potential of butterfly pea for breeding programs, especially with the identified genetic diversity and distinct characteristics in the Purple Double germplasm.