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<p>Lipases are a group of industrially important enzymes widely used in food processing, pharmaceuticals, fine chemicals, etc., due to their ability to catalyze the hydrolysis of fats and oils into diglycerides, monoglycerides, glycerol, and free fatty acids. However, the high cost of enzyme production remains a significant challenge, leading to the search for economical production methods. One promising approach is using low-cost agro-industrial residues as substrates, which reduces production costs and supports waste valorization. However, data on optimal conditions for lipase production by <i>Rhizopus</i> sp. using such residues remain limited. Therefore, the present study aimed to determine the optimum parameters favorable for lipase production by <i>Rhizopus</i> sp. employing low-cost agro-industrial residues under submerged fermentation and to partially purify the enzyme. Deoiled sesame cake (SC) was found to be the best substrate among four readily available residues evaluated. Optimization was carried out using the one-variable-at-a-time approach by varying temperature, pH, incubation period, inoculum size, and agitation. The optimum conditions for maximum lipase production were 35&#xa0;°C, pH 8, 96&#xa0;h of incubation, 1% (v/v) inoculum, and shaking at 100&#xa0;rpm. Glucose, peptone, Triton X-100, tributyrin, and olive oil were found to be the best carbon source, nitrogen source, surfactant, fat, and oil, respectively. Under the optimum conditions, the strain showed a maximum of 7.84&#xa0;U/ml of lipase activity. The enzyme was purified by ammonium sulfate precipitation followed by ion exchange chromatography. On SDS PAGE, the purified fraction showed a single band with a molecular weight of ~ 40&#xa0;kDa.</p>

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Enhanced production and partial purification of lipase from Rhizopus sp. grown on low-cost deoiled sesame cake

  • 期刊论文

  • 发表日期：

    2025年6月5日

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  • S. Priya,
  • R. Yuvaraj,
  • S. Mrudula

摘要

Lipases are a group of industrially important enzymes widely used in food processing, pharmaceuticals, fine chemicals, etc., due to their ability to catalyze the hydrolysis of fats and oils into diglycerides, monoglycerides, glycerol, and free fatty acids. However, the high cost of enzyme production remains a significant challenge, leading to the search for economical production methods. One promising approach is using low-cost agro-industrial residues as substrates, which reduces production costs and supports waste valorization. However, data on optimal conditions for lipase production by Rhizopus sp. using such residues remain limited. Therefore, the present study aimed to determine the optimum parameters favorable for lipase production by Rhizopus sp. employing low-cost agro-industrial residues under submerged fermentation and to partially purify the enzyme. Deoiled sesame cake (SC) was found to be the best substrate among four readily available residues evaluated. Optimization was carried out using the one-variable-at-a-time approach by varying temperature, pH, incubation period, inoculum size, and agitation. The optimum conditions for maximum lipase production were 35 °C, pH 8, 96 h of incubation, 1% (v/v) inoculum, and shaking at 100 rpm. Glucose, peptone, Triton X-100, tributyrin, and olive oil were found to be the best carbon source, nitrogen source, surfactant, fat, and oil, respectively. Under the optimum conditions, the strain showed a maximum of 7.84 U/ml of lipase activity. The enzyme was purified by ammonium sulfate precipitation followed by ion exchange chromatography. On SDS PAGE, the purified fraction showed a single band with a molecular weight of ~ 40 kDa.